人参皂苷Rg1参与诱导小鼠多潜能干细胞的研究

被引：3

作者：

胡文涛 ^{[1
]}

闫秋月 ^{[2
]}

方瑜 ^{[1
]}

邱占东 ^{[1
]}

张苏明 ^{[1
]}

机构：

[1] 华中科技大学同济医学院附属同济医院神经内科

[2] 沧州市中心医院神经内科

来源：

神经损伤与功能重建 | 2014年 / 9卷 / 03期

基金：

中国博士后科学基金;

关键词：

人参皂苷Rg1; 诱导多潜能干细胞; 细胞重编程; 鼠胚成纤维细胞;

D O I：

暂无

中图分类号：

R329.2 [人体细胞学];

学科分类号：

100107 [人体解剖与组织胚胎学（人体解剖学、组织与胚胎学）];

摘要：

目的:观察人参皂苷Rg1对鼠胚成纤维细胞(MEF)向诱导多潜能干细胞(iPSCs)诱导转化效率的影响。方法:采用经典四因子的逆转录病毒载体感染,在细胞感染后3 d内在MEF培养基里加入人参皂苷Rg1(0、0.3、1、3、10μg/mL),进行iPSCs诱导及鉴定。结果:人参皂苷Rg1(1μg/mL)组可明显提高iPSCs的诱导效率,诱导效率为(0.0450±0.0019)%,人参皂苷Rg1(0μg/mL)组为(0.0100±0.0033)%,所获iPSCs经鉴定为阳性。结论:人参皂苷Rg1可能在提高小鼠iPSCs的诱导效率方面发挥一定作用。

引用

页码：184 / 186

页数：3

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