Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector

被引：184

作者：

Gonzalez, Federico ^{[1
]}

Barragan Monasterio, Montserrat ^{[1
]}

Tiscornia, Gustavo ^{[1
]}

Montserrat Pulido, Nuria ^{[1
]}

Vassena, Rita ^{[1
]}

Batlle Morera, Laura ^{[1
]}

Rodriguez Piza, Ignasi ^{[1
]}

Izpisua Belmonte, Juan Carlos ^{[1
,2
]}

机构：

[1] Ctr Regenerat Med Barcelona, Barcelona 08003, Spain

[2] Salk Inst Biol Studies, La Jolla, CA 92037 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 22期

基金：

瑞士国家科学基金会;

关键词：

iPS cells; nonintegrative technique; reprogramming; pluripotency; HUMAN SOMATIC-CELLS; FIBROBLASTS; INDUCTION; EFFICIENT; ABSENCE; SYSTEM; OCT4; MYC;

D O I：

10.1073/pnas.0901471106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Induced pluripotent stem (iPS) cells have generated keen interest due to their potential use in regenerative medicine. They have been obtained from various cell types of both mice and humans by exogenous delivery of different combinations of Oct4, Sox2, Klf4, c-Myc, Nanog, and Lin28. The delivery of these transcription factors has mostly entailed the use of integrating viral vectors ( retroviruses or lentiviruses), carrying the risk of both insertional mutagenesis and oncogenesis due to misexpression of these exogenous factors. Therefore, obtaining iPS cells that do not carry integrated transgene sequences is an important prerequisite for their eventual therapeutic use. Here we report the generation of iPS cell lines from mouse embryonic fibroblasts with no evidence of integration of the reprogramming vector in their genome, achieved by nucleofection of a polycistronic construct coexpressing Oct4, Sox2, Klf4, and c-Myc.

引用

页码：8918 / 8922

页数：5

共 27 条

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