MMP-1、MMP-3和MMP-13在慢性睡眠剥夺所致颞下颌关节损伤中的变化

被引：3

作者：

吕晓伟 ^{[1
,2
]}

吴高义 ^{[1
]}

何玉宏 ^{[1
]}

王培欢 ^{[1
]}

朱国雄 ^{[1
]}

机构：

[1] 济南军区总医院口腔科

[2] 辽宁医学院

来源：

现代生物医学进展 | 2015年 / 15卷 / 27期

基金：

中国博士后科学基金;

关键词：

慢性睡眠剥夺; 颞下颌关节; 颞下颌关节紊乱病; 基质金属蛋白酶(MMPs); 关节结构;

D O I：

10.13241/j.cnki.pmb.2015.27.004

中图分类号：

R782.6 [颞下颌关节疾病];

学科分类号：

100213 [耳鼻咽喉科学];

摘要：

目的:观察MMP-1、MMP-3和MMP-13在慢性睡眠剥夺所致颞下颌关节损伤中表达的变化,探讨慢性睡眠剥夺所致颞下颌关节损伤的可能机制。方法:采用改良多平台(MMPM)建立大鼠慢性睡眠剥夺模型,将90只成年雄性Wastar大鼠随机分为小平台组、网格组和对照组。小平台组和网格组大鼠接受每天18 h的睡眠剥夺和6 h间歇期(10:00-16:00),间歇期大鼠正常笼养。实验第7、14和21 d时分别观察动物的行为学观察、检测动物血浆皮质醇(CORT)和促肾上腺皮质激素(ACTH)水平检测,通过免疫印迹法和实时定量聚合酶链反应(PRC)检测颞下颌关节软骨中MMP-1、MMP-3和MMP-13的蛋白和m RNA表达,并通过HE染色法观察颞下颌关节结构的变化。结果:与对照组和网格组大鼠相比,小平台组大鼠第14 d和21 d时髁突软骨中间部位表面纤维在出现明显的炎症、松解及脱落现象;第21天时的血浆ACTH和CORT水平均显著高于网格组和对照组,差异有统计学意义(P<0.05);第7、14、21 d时关节软骨MMP-1和MMP-13蛋白和m RNA的表达水平均显著上调(P<0.05)。结论:慢性睡眠剥夺所致的颞下颌关节损伤可能与关节软骨中MMP-1、MMP-3和MMP-13的表达上调有关。

引用

页码：5212 / 5215

页数：4

共 15 条

[1]

Effects of sleep deprivation on pain-related factors in the temporomandibular joint [J].

Wu, Gaoyi ;

Chen, Lei ;

Wei, Gang ;

Li, Ying ;

Zhu, Guoxiong ;

Zhao, Zhengwei ;

Huang, Fei .

JOURNAL OF SURGICAL RESEARCH, 2014, 192 (01) :103-111

[2]

Effects of deer bone extract on the expression of pro-inflammatory cytokine and cartilage-related genes in monosodium iodoacetate-induced osteoarthritic rats [J].

Lee, Hyunji ;

Choi, Hyeon-Son ;

Park, Yooheon ;

Ahn, Chang Won ;

Jung, Sung Ug ;

Park, Soo Hyun ;

Suh, Hyung Joo .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2014, 78 (10) :1703-1709

[3]

The Effects of Amphiregulin Induced MMP-13 Production in Human Osteoarthritis Synovial Fibroblast.[J].Yi-Te Chen;Chun-Han Hou;Sheng-Mou Hou;Ju-Fang Liu;Luca Cantarini.Mediators of Inflammation.2014,

[4]

A hyaluronic acid–salmon calcitonin conjugate for the local treatment of osteoarthritis: Chondro-protective effect in a rabbit model of early OA.[J].Anna Mero;Monica Campisi;Marta Favero;Carlo Barbera;Cynthia Secchieri;Jean M. Dayer;Mary B. Goldring;Steven R. Goldring;Gianfranco Pasut.Journal of Controlled Release.2014,

[5]

Influence of sleep deprivation on expression of MKK4 and c-fos in the mandibular condylar cartilage of rats.[J].Jinlong Chen;Gaoyi Wu;Guoxiong Zhu;Peihuan Wang;Hongyu Chen;Huaqiang Zhao.British Journal of Oral & Maxillofacial Surgery.2013,

[6]

Prolonged matrix metalloproteinase-3 high expression after cyclic compressive load on human synovial cells in three-dimensional cultured tissue.[J].Y. Akamine;K. Kakudo;M. Kondo;K. Ota;Y. Muroi;H. Yoshikawa;K. Nakata.International Journal of Oral & Maxillofacial Surgery.2011, 7

[7]

Evidence for indirect effects of pain catastrophizing on clinical pain among myofascial temporomandibular disorder participants: The mediating role of sleep disturbance [J].

Buenaver, Luis F. ;

Quartana, Phillip J. ;

Grace, Edward G. ;

Sarlani, Eleni ;

Simango, Mpepera ;

Edwards, Robert R. ;

Haythornthwaite, Jennifer A. ;

Smith, Michael T. .

PAIN, 2012, 153 (06) :1159-1166

[8]

Psychological stress induces alterations in temporomandibular joint ultrastructure in a rat model of temporomandibular disorder [J].

Wu, Gaoyi ;

Chen, Lei ;

Zhu, Guoxiong ;

Su, Yucheng ;

Chen, Yongjin ;

Sun, Jing ;

Wang, Yanliang .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2011, 112 (06) :E106-E112

[9]

Changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in the condylar cartilage of rats induced by experimentally created disordered occlusion [J].

Wang, Guo-Wei ;

Wang, Mei-Qing ;

Wang, Xiao-Jing ;

Yu, Shi-Bin ;

Liu, Xiao-Dong ;

Jiao, Kai .

ARCHIVES OF ORAL BIOLOGY, 2010, 55 (11) :887-895

[10]

MMP-3 activation is a hallmark indicating an early change in TMJ disorders; and is related to nitration.[J].H. Fujita;T. Morisugi;Y. Tanaka;T. Kawakami;T. Kirita;Y. Yoshimura.International Journal of Oral & Maxillofacial Surgery.2008, 1

← 1 2 →