跑台运动对骨质疏松大鼠骨组织Wnt/β-catenin信号通路的影响

被引：13

作者：

黄晓 ^{[1
]}

李建华 ^{[2
]}

机构：

[1] 杭州, 浙江大学城市学院

[2] 浙江大学附属邵逸夫医院

来源：

中华物理医学与康复杂志 | 2018年 / 40卷 / 10期

关键词：

运动; 骨质疏松; Wnt/β-catenin; 信号通路;

D O I：

暂无

中图分类号：

R580 [];

学科分类号：

摘要：

目的研究跑台运动对骨质疏松大鼠骨代谢的影响以及Wnt/β-catenin信号通路在运动影响骨代谢中的调节作用。方法选取2月龄SD雌性大鼠32只, 按随机数字表法分为假手术安静组(S组)、去卵巢安静组(O组)、假手术运动组(ST组)和去卵巢运动组(OT组), 每组大鼠8只。采用去卵巢手术制造大鼠绝经后骨质疏松模型, O组和OT组均摘除卵巢, S组和ST组大鼠的手术只进行到找到卵巢, 但并不摘除卵巢。造模成功后, S组和O组大鼠在饲养笼中自由活动, ST组和OT组大鼠除在饲养笼中自由活动外, 每日还进行跑台训练。于ST组和OT组最后1次运动结束24 h后(入组10周后)处死4组大鼠, 分别采用双能X线骨密度仪测定大鼠股骨骨密度(BMD), 全自动生化分析仪检测血清钙(Ca)、磷(P)、碱性磷酸酶(ALP)含量, 放射免疫法检测血清骨钙素(BGP)活性, 三点弯曲试验检测股骨生物力学指标, Western blot测定骨组织Wnt/β-catenin信号通路相关蛋白的表达。结果入组10周后, O组大鼠BMD显著低于S组大鼠, ST组大鼠BMD显著高于S组大鼠, OT组大鼠BMD显著高于O组大鼠BMD, 组间差异均有统计学意义(P<0.05);ST组大鼠Wnt/β-catenin信号通路蛋白P-GSK-3β的相对表达量为(0.51±0.06), 显著低于S组的(0.80±0.05), 组间差异有统计学意义(P<0.05), 而ST组大鼠β-catenin和cyclin D1的相对表达量均显著高于S组, 组间差异均有统计学意义(P<0.05);OT组大鼠Wnt/β-catenin信号通路蛋白P-GSK-3β的相对表达量显著低于O组, 而β-catenin和cyclin D1的相对表达量则显著高于O组, 组间差异均有统计学意义(P<0.05)。结论跑台运动可显著改善骨质疏松大鼠的骨代谢, 其机制可能与Wnt/β-catenin信号通路的激活相关。

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