里氏木霉表达异源中性内切纤维素酶蛋白以改善最佳酶活pH

被引：4

作者：

朱姚

孟凡举

吕丹丹

朱亚然

赵喜华

魏东芝

王玮

机构：

[1] 华东理工大学鲁华所

来源：

工业微生物 | 2014年 / 44卷 / 05期

关键词：

里氏木霉; 中性纤维素酶; 接合转移; cbh1启动子;

D O I：

暂无

中图分类号：

Q936 [微生物生物化学];

学科分类号：

071005 [微生物学];

摘要：

为了提高里氏木霉中天然纤维素酶的最佳活性pH,本实验从特异腐质霉,灰腐质霉的变种,枯草芽孢杆菌LH中分别筛选并克隆了其含有的中性纤维素酶基因,将其置于里氏木霉cbh1启动子的启动下,在里氏木霉中进行异源表达。改造株在pH 6.0下酶活提升16%,pH 7.0下活性保留75%以上,而此时原始菌酶活残留20%。本实验所得的产中性纤维素酶里氏木霉基因改造株,由于其良好的中酸性活性表现,在食品,纺织,纸浆和造纸行业应也有良好的使用潜力。

引用

页码：37 / 41

页数：5

共 9 条

[1]

A comparative study of β-1; 4-endoglucanase (possessing β-1; 4-exoglucanase activity) from Bacillus subtilis LH expressed in Pichia pastoris GS115 and Escherichia coli Rosetta (DE3).[J].Xi-Hua Zhao;Wei Wang;Feng-Qing Wang;Dong-Zhi Wei.Bioresource Technology.2011,

[2]

Construction of two vectors for gene expression in Trichoderma reesei.[J].Dandan Lv;Wei Wang;Dongzhi Wei.Plasmid.2011, 1

[3]

High efficient expression of cellobiase gene from Aspergillus niger in the cells of Trichoderma reesei [J].

Wang, Bingbing ;

Xia, Liming .

BIORESOURCE TECHNOLOGY, 2011, 102 (06) :4568-4572

[4]

Agrobacterium-mediated transformation as a tool for functional genomics in fungi [J].

Michielse, CB ;

Hooykaas, PJJ ;

van den Hondel, CAMJJ ;

Ram, AFJ .

CURRENT GENETICS, 2005, 48 (01) :1-17

[5]

Purification and characterisation of alkaline cellulase produced by a novel isolate; Bacillus sphaericus JS1.[J].Jagtar Singh;Navneet Batra;Ranbir Chander Sobti.Journal of Industrial Microbiology & Biotechnology.2004, 2

[6]

Enzymatic properties, crystallization, and deduced amino acid sequence of an alkaline endoglucanase from Bacillus circulans [J].

Hakamada, Y ;

Endo, K ;

Takizawa, S ;

Kobayashi, T ;

Shirai, T ;

Yamane, T ;

Ito, S .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2002, 1570 (03) :174-180

[7]

Efficient Agrobacterium tumefaciens-mediated gene disruption in the phytopathogen Mycosphaerella graminicola [J].

Zwiers, LH ;

De Waard, MA .

CURRENT GENETICS, 2001, 39 (5-6) :388-393

[8]

Crystal structure of alkaline cellulase K: insight into the alkaline adaptation of an industrial enzyme 1 1 Edited by R. Huber.[J].Tsuyoshi Shirai;Hirokazu Ishida;Jun-ichi Noda;Takashi Yamane;Katsuya Ozaki;Yoshihiro Hakamada;Susumu Ito.Journal of Molecular Biology.2001, 5

[9]

Enzymatic properties of cellulases from Humicola insolens.[J].Martin Schülein.Journal of Biotechnology.1997, 1

← 1 →