基于超高效液相色谱-质谱红花药材指纹图谱的建立

被引：5

作者：

罗春霞

王瑾

祁艳茹

马丽

杨亮

关明

机构：

[1] 新疆师范大学化学化工学院

来源：

分析测试技术与仪器 | 2019年 / 25卷 / 03期

关键词：

超高效液相色谱-质谱; 红花药材; 指纹图谱;

D O I：

10.16495/j.1006-3757.2019.03.003

中图分类号：

O657.63 [质谱分析]; R284.1 [化学分析与鉴定];

学科分类号：

070302 [分析化学]; 100804 [中药化学];

摘要：

以红花为研究对象,建立超高效液相色谱-质谱（UPLC-MS）指纹图谱分析方法,为红花药材质量鉴别提供借鉴.通过考察检测波长、梯度洗脱条件、提取溶剂、提取方法等因素,在最优条件下对20个产地的红花样品进行分离分析.色谱柱:Thermo Hypersil Gold C18,100 mm×2.1 mm,1.9μm,流动相:0.1%甲酸水溶液（A）-乙腈（B）;流速:0.3 mL/min;柱温:30℃;检测波长:270 nm;进样量:2μL,质谱条件:鞘气流速:45 arb,辅助气流速15 arb,雾化电压:3.3 KV,离子传输管温度:350℃,辅助气加热温度:400℃.试验结果:共确定了67个共有峰,通过UPLC-MS指认了其中11个共有峰,对20批不同产地的红花药材指纹图谱进行了相似度评价,其相似度在0.687～0.971之间.建立的UPLC-MS指纹图谱可为红花药材的质量控制提供参考依据.

引用

页码：152 / 159

页数：8

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