By using orthogonal design, the variation in chemical composition contents of \%Dendrocalamus latiflorus \%Munro culm\|wood were systematically analyzed with different sites, ages, planting modes, positions. The results showed that age and position of bamboo (including vertical position and horizonal position) are the main factors which affect their chemical composition contents, while site and planting mode are insignificant ones. The orders of degree to different factors affecting the chemical composition are different.