类胡萝卜素生物合成途径及其控制与遗传操作

被引:30
作者
赵文恩
李艳杰
崔艳红
乔宪生
不详
机构
[1] 中国农业科学院郑州果树研究所
[2] 郑州大学化工学院
[3] 中国农业科学院郑州果树研究所 郑州
[4] 郑州
关键词
类胡萝卜素生物合成途径; 生物合成酶; 合成途径控制; 基因转化;
D O I
暂无
中图分类号
Q946 [植物生物化学];
学科分类号
071001 ;
摘要
类胡萝卜素在真菌和植物细胞胞液/内质网上是由乙酰CoA经甲羟戊酸途径合成的,在细菌与植物质体中由磷酸甘油醛与丙酮酸经1-脱氧木酮糖-5-磷酸途径合成.形成的异戊烯基焦磷酸经多次缩合生成第一个类胡萝卜素八氢番茄红素,再经脱氢、环化、羟基化、环氧化等转变为其它类胡萝卜素.类胡萝卜素生物合成中涉及的酶都是膜结合的或整合入膜中的.类胡萝卜素合成是通过底物可利用性与环化分支方式进行控制的.白色体到叶绿体的转变以及花与果实成熟时类胡萝卜素合成增加是在基因转录水平调节的.进行类胡萝卜素合成酶基因的转化,可增加转化体类胡萝卜素的积累.
引用
收藏
页码:930 / 942
页数:13
相关论文
共 14 条
[1]  
Antisense inhibition of the beta-carotene hydroxylase enzyme in Arabidopsis and the implications for carotenoid accumulation, photoprotection and antenna assembly[J] . Heather M. Rissler,Barry J. Pogson.Photosynthesis Research . 2001 (1)
[2]   Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production [J].
Kim, SW ;
Keasling, JD .
BIOTECHNOLOGY AND BIOENGINEERING, 2001, 72 (04) :408-415
[3]  
Phytoene synthase from tomato (Lycopersicon esculentum) chloroplasts – partial purification and biochemical properties[J] . Paul D. Fraser,Wolfgang Schuch,Peter M. Bramley.Planta . 2000 (3)
[4]   Structural and functional analysis of the gene cluster encoding carotenoid biosynthesis in Mycobacterium aurum A+ [J].
Viveiros, M ;
Krubasik, P ;
Sandmann, G ;
Houssaini-Iraqui, M .
FEMS MICROBIOLOGY LETTERS, 2000, 187 (01) :95-101
[5]   A carotenogenic gene cluster from Brevibacterium linens with novel lycopene cyclase genes involved in the synthesis of aromatic carotenoids [J].
Krubasik, P ;
Sandmann, G .
MOLECULAR AND GENERAL GENETICS, 2000, 263 (03) :423-432
[6]   Metabolic engineering of carotenoid accumulation in Escherichia coli by modulation of the isoprenoid precursor pool with expression of deoxyxylulose phosphate synthase [J].
Matthews, PD ;
Wurtzel, ET .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2000, 53 (04) :396-400
[7]   Phytoene synthase-2 enzyme activity in tomato does not contribute to carotenoid synthesis in ripening fruit [J].
Fraser, PD ;
Kiano, JW ;
Truesdale, MR ;
Schuch, W ;
Bramley, PM .
PLANT MOLECULAR BIOLOGY, 1999, 40 (04) :687-698
[8]  
Xanthophyll biosynthesis: molecular and functional characterization of carotenoid hydroxylases from pepper fruits ( Capsicum annuum L.)[J] . Florence Bouvier,Yves Keller,Alain d’Harlingue,Bilal Camara.Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism . 1998 (3)
[9]   Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts [J].
Misawa, N ;
Shimada, H .
JOURNAL OF BIOTECHNOLOGY, 1998, 59 (03) :169-181
[10]  
Phytoene Desaturase: Heterologous Expression in an Active State, Purification, and Biochemical Properties[J] . Protein Expression and Purification . 1997 (2)