响应曲面分析法优化苹果渣中多酚类物质的果胶酶辅助提取工艺

被引:28
作者
崔春兰 [1 ,2 ]
郑虎哲 [1 ,2 ]
顾立众 [1 ,2 ]
CHUNG Shinkyo [3 ]
机构
[1] 江苏食品加工技术研发中心
[2] 江苏食品药品职业技术学院食品与营养工程学院
[3] 大韩民国国立庆北大学校食品工学科
关键词
苹果渣; 总多酚; 咖啡酸; 果胶酶; 响应面分析法;
D O I
10.13982/j.mfst.1673-9078.2013.09.047
中图分类号
TS209 [食品工业副产品加工与利用];
学科分类号
083203 [农产品加工及贮藏工程];
摘要
研究了响应面分析法对苹果渣中多酚类物质的果胶酶辅助提取工艺。以总多酚、咖啡酸提取量为评价指标,在单因素实验基础上根据中心组合实验设计原理,采用3因素5水平的响应曲面分析法对提取工艺进行了优化。通过响应曲面实验及回归分析得出最佳工艺条件为:果胶酶/基质比0.11、反应温度38.00℃、反应时间12.53 h。在此条件下,预测总多酚和咖啡酸提取量分别为11.32g GAE/kg和173.87 mg/kg。经验证,总多酚和咖啡酸提取量分别达到11.08 g GAE/kg和170.03 mg/kg。另外,相对于对照组的6.78 g GAE/kg和13.14 mg/kg分别提高1.6倍和12.9倍。表明由响应曲面法获得的二次模型方程能较好地预测实验结果及优化条件;果胶酶辅助提取工艺稳定合理、准确可靠,是提取苹果渣中总多酚、咖啡酸的可行方法。
引用
收藏
页码:2235 / 2240
页数:6
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