枸杞多糖诱导人脐血间充质干细胞向神经元样细胞分化的实验研究

被引：6

作者：

陈晓岚

陈芬

莫艳秀

汤银娟

机构：

[1] 湘南学院组织学与胚胎学教研室

来源：

现代生物医学进展 | 2007年 / 08期

关键词：

人脐血; 间充质干细胞; 枸杞多糖; 诱导分化; 神经元样细胞;

D O I：

10.13241/j.cnki.pmb.2007.08.009

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的:探讨枸杞多糖诱导人脐血间充质干细胞(MSCs)向神经元样细胞分化的可行性及其机制。方法:无菌条件下收集正常足月儿的脐带血,经肝素抗凝,用相对密度1.077的淋巴细胞分离液分离脐血的单个核细胞,用低糖DMEM培养基进行培养和纯化扩增。选取第3代细胞进行诱导实验,当传代细胞长满瓶底的80%以上时,先用含15%FBS和10ng/ml bFGF的DMEM完全培养基预诱导24小时,然后用不含血清含1g/L枸杞多糖的DMEM培养基诱导,光镜下观察细胞形态,用免疫组化技术检测细胞Nestin和NF的表达。结果:预诱导后MSCs没有变化,而经枸杞多糖诱导4h后细胞即出现形态学上的改变,细胞变成不规则形,立体感增强,从胞体伸出突起。免疫组化检测显示,细胞Nestin、NF呈阳性。结论:人脐血间充质干细胞经枸杞多糖诱导可转化为神经元样细胞,其诱导机制可能与枸杞多糖的抗氧化作用有关。

引用

页码：1174 / 1175+1289+1173 +1289

页数：4

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