黄芪甲苷后处理对乳鼠心肌细胞缺氧复氧损伤的作用研究

被引：5

作者：

刘辉

王宁

梁宏亮

苏洁

俞世强

刘金成

易定华

机构：

[1] 第四军医大学西京医院心血管外科

来源：

现代生物医学进展 | 2014年 / 14卷 / 17期

关键词：

黄芪甲苷; 乳鼠心肌细胞; 超氧化物歧化酶; 丙二醛; 心肌保护;

D O I：

10.13241/j.cnki.pmb.2014.17.006

中图分类号：

Q95-3 [动物学的研究与实验]; R285.5 [中药实验药理];

学科分类号：

摘要：

目的:观察黄芪甲苷(AstragalosideⅣ,AsⅣ)后处理对缺氧复氧损伤(simulated ischemia reperfusion injury,SI/RI)的SD乳鼠心肌细胞是否具有保护作用。方法:将乳鼠原代心肌细胞平均分为五组,即空白对照组(Control)、缺氧复氧处理组(SI/RI)、黄芪甲苷预处理(5,10、20μM)+SI/RI组(AsIV+SI/RI)。各组细胞经处理后,四氮唑溴盐比色法(MTT)检测各组细胞存活率;TUNEL染色法测定各组细胞凋亡率;SOD测试盒检测培养液中超氧化物歧化酶(SOD)含量,总嘌呤氧化酶(XOD)测试盒检测丙二醛(MDA)含量。Western blot法检测各组细胞抗凋亡蛋白Bcl-2和促凋亡蛋白Caspase-3的表达。结果:与空白组相比,缺氧复氧损伤组细胞活力显著下降(P<0.05),凋亡率显著上升(P<0.05),其培养液中SOD水平显著降低(P<0.05),MDA水平显著升高。而不同浓度AsⅣ后处理组的心肌细胞存活率显著上升,凋亡率显著下降,培养液中SOD水平显著上升,MDA水平显著下降(P<0.05),且呈浓度呈依赖性。Western blot结果显示AsⅣ后处理组细胞中的Bcl-2表达明显上升,Caspase-3明显下降。结论:黄芪甲苷后处理对缺氧复氧诱导的乳鼠心肌细胞损伤具有显著的保护作用,能够显著上调抗凋亡蛋白Bcl-2的表达,下调促凋亡蛋白Caspase-3的表达。

引用

页码：3223 / 3227

页数：5

共 12 条

[1] Pharmacological effects of Astragaloside IV: a literature review
Shuang Ren
Hua Zhang
Yongping Mu
Mingyu Sun
Ping Liu
[J]. Journal of Traditional Chinese Medicine, 2013, 33 (03) : 413 - 416
[2] 黄芪皂苷Ⅳ对缺血大鼠心肌钙运转和心功能的影响(英文)
李自普
曹倩
[J]. Acta Pharmacologica Sinica, 2002, (10) : 36 - 42
[3] Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression[J] . Dingkun Gui,Jianhua Huang,Yongping Guo,Jianguo Chen,Yifang Chen,Wenzhen Xiao,Xusheng Liu,Niansong Wang. Cytokine . 2013
[4] Genetic and Pharmacological Inhibition of Rheb1-mTORC1 Signaling Exerts Cardioprotection against Adverse Cardiac Remodeling in Mice
Wu, Xiangqi
Cao, Yunshan
Nie, Junwei
Liu, Hailang
Lu, Shuangshuang
Hu, Xiaoshan
Zhu, Jingai
Zhao, Xia
Chen, Jiandong
Chen, Xiaohu
Yang, Zhongzhou
Li, Xinli
[J]. AMERICAN JOURNAL OF PATHOLOGY, 2013, 182 (06) : 2005 - 2014
[5] Hypoxia-Inducible Factors in Physiology and Medicine
Semenza, Gregg L.
[J]. CELL, 2012, 148 (03) : 399 - 408
[6] Astragaloside IV protects against focal cerebral ischemia/reperfusion injury correlating to suppression of neutrophils adhesion-related molecules
Li, Min
Qu, You Z.
Zhao, Zhen W.
Wu, Sheng X.
Liu, Ying Y.
Wei, Xiao Y.
Gao, Li
Gao, Guo D.
[J]. NEUROCHEMISTRY INTERNATIONAL, 2012, 60 (05) : 458 - 465
[7] New Horizons in Cardioprotection: Recommendations From the 2010 National Heart, Lung, and Blood Institute Workshop[J] . Lisa Schwartz Longacre,Robert A. Kloner,Andrew E. Arai,Christopher P. Baines,Roberto Bolli,Eugene Braunwald,James Downey,Raymond J. Gibbons,Roberta A. Gottlieb,Gerd Heusch,Robert B. Jennings,David J. Lefer,Robert M. Mentzer,Elizabeth Murphy,Michel Ovize,Peipei Ping,Karin Przyklenk,Michael N. Sack,Richard S. Vander Heide,Jakob Vinten-Johansen,Derek M. Yellon. Circulation
[8] Astragaloside IV Protects Against Ischemia Reperfusion in a Murine Model of Orthotopic Liver Transplantation
Cheng, M. -X.
Chen, Z. -Z.
Cai, Y. -L.
Liu, C. -A.
Tu, B.
[J]. TRANSPLANTATION PROCEEDINGS, 2011, 43 (05) : 1456 - 1461
[9] The healing and anti-scar effects of astragaloside IV on the wound repair in vitro and in vivo[J] . Xi Chen,Li-Hua Peng,Ni Li,Qi-Mei Li,Ping Li,Kwok-Pui Fung,Ping-Chung Leung,Jian-Qing Gao. Journal of Ethnopharmacology . 2011 (3)
[10] Cardioprotection: Nitric Oxide, Protein Kinases, and Mitochondria[J] . Gerd Heusch,Kerstin Boengler,Rainer Schulz. Circulation . 2008 (19)

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