Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29

被引：12

作者：

Jing LI Qian YANG Lihua ZHAO Shumei ZHANG Yuxia WANG Xiaoyu ZHAO Department of Life Science and Engineering Harbin Institute of Technology Harbin China Institute of Microbiology of Heilongjiang Academy of Sciences Harbin China Heilongjiang University Press Harbin China ^{[1
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机构：

来源：

Journal of Zhejiang University(Science B:An International Biomedicine & Biotechnology Journal) | 2009年 / 10卷 / 04期

关键词：

Bacillus subtilis; Antifungal protein; Purification;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl (DEAE)-52 cellulose and gel filtration chromatography on Bio-Gel? P-100. The protein was absorbed on DEAE-cellulose and Bio-Gel? P-100. The purified antifungal fraction was designated as B29I, with a molecular mass of 42.3 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), pI value 5.69 by isoelectric focusing (IEF)-PAGE, and 97.81% purity by high performance liquid chromatography (HPLC). B29I exhibited inhibitory activity on mycelial growth in Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme, and Sclerotinia sclerotiorum. The 50% inhibitory concentrations (IC50) of its antifungal activity toward Fusarium oxysporum and Rhizoctonia solani were 45 and 112 μmol/L, respectively. B29I also demonstrated an inhibitory effect on conidial spore germination of Fusarium oxysporum and suppression of germ-tube elongation, and induced distortion, tumescence, and rupture of a portion of the germinated spores.

引用

页码：264 / 272

页数：9

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