生物炭添加对秸秆还田土壤细菌群落结构和多样性影响

被引：26

作者：

张玉洁

吴婷

赵娟

刘书路

于跃刚

张浩

机构：

[1] 安徽师范大学环境科学与工程学院

来源：

环境科学学报 | 2017年 / 37卷 / 02期

关键词：

秸秆还田; 生物炭; 细菌群落; 多样性; DGGE;

D O I：

10.13671/j.hjkxxb.2016.0333

中图分类号：

S154.3 [土壤微生物学];

学科分类号：

071012 ; 0713 ;

摘要：

为探讨生物炭对秸秆还田土壤细菌群落结构和多样性的影响,采用变性梯度凝胶电泳(PCR-DGGE)技术结合克隆测序技术,对未添加(SW)和添加(SBW)生物炭秸秆还田土壤细菌群落结构和多样性进行表征.DGGE谱图和多样性分析结果表明,生物炭添加增加了秸秆还田土壤细菌DGGE图谱条带位置和灰度值及均匀度的变化,但两个处理土壤细菌群落Shannon-Wiener指数和丰富度没有显著差异(p>0.05).聚类分析结果表明,SBW处理土壤细菌群落聚为5类,SW处理土壤细菌群落可聚为6类.DGGE条带测序和结构分析结果表明,生物炭添加导致秸秆还田土壤中Actinobacteria、Nitrospira消失和Spirochaetes、Gemmatimonadetes、Chloroflexi、WS3出现,并导致Firmicutes、Proteobacteria所占比例升高和Acidobacteria、Bacteroides所占比例降低,说明生物炭添加促进了秸秆还田土壤细菌群落组成结构变化.

引用

页码：712 / 720

页数：9

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