赤霉菌原生质体DNA转化

被引：7

作者：

朱平

杨金玲

朱慧新

程克棣

机构：

[1] 中国医学科学院

[2] 中国协和医科大学

[3] 药物研究所卫生部天然药物生物合成重点实验室!北京

来源：

菌物系统 | 2000年 / 02期

关键词：

赤霉菌; 原生质体; DNA转化; 潮霉素B抗性基因;

D O I：

10.13346/j.mycosystema.2000.02.024

中图分类号：

Q943 [植物细胞遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Methods for the protoplast preparation and DNA transformation of Gibberella fujikuroi were established. The protoplasts were transformed with plasmid pAN7-1, which carries hygromycin B resistant gene (hPh), and the transformation frequency was about 10. Some transformants grew vigorously on the selectivemedium containing 400μg / ml of hygromycin B, while the minimum inhibitory concentration (MIC) of the antibiotic to the recipient strain was oniy 20μg / ml. Southern hybridization showed that the hph gene has integrated into the genomic DNA of the recipient strain in different manners.

引用

页码：283 / 287

页数：5

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