磁刺激对体外原代神经元迁移的影响

被引：1

作者：

杨云凤

吴碧华

机构：

[1] 川北医学院附属医院老年科

来源：

南昌大学学报(医学版) | 2014年 / 54卷 / 02期

关键词：

磁刺激; 原代神经元; transwell小室; 体外; 大鼠;

D O I：

10.13764/j.cnki.ncdm.2014.02.006

中图分类号：

R741 [神经病学];

学科分类号：

100204 [神经病学];

摘要：

目的体外观察磁刺激对原代神经元迁移的影响。方法原代培养SD大鼠皮质神经元,分为正常组(control,C组)、假刺激组(shame,S组)、40%最大输出强度组(40%of maximum intensity of stimulation,0.4T组)、60%最大输出强度组(60%of maximum intensity of stimulation,0.6T组)。各组细胞接种于transwell小室内,自细胞接种后第2～6天接受磁刺激,连续刺激5d,各组细胞于第6天同一时间染色,将transwell小室倒置,光学显微镜下计数。结果 C组有(2.6±0.548)个、S组有(3.0±0.707)个细胞迁移,2组比较差异无统计学意义(P>0.05);0.4T组有(17.40±1.673)个、0.6T组有(18.40±1.572)个细胞迁移,迁移的数量较C组及S组明显增多(P<0.05)。结论磁刺激可促进体外原代神经元迁移。

引用

页码：20 / 23+107 +107

页数：5

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