SELEX技术及寡核苷酸适配体的近期研究进展

被引：10

作者：

郭磊

机构：

[1] 军事医学科学毒物药物研究所八室

来源：

国际药学研究杂志 | 2010年 / 37卷 / 04期

关键词：

指数富集的配体系统进化; 寡核苷酸适配体; 肿瘤靶向; 生物医学; 药物学;

D O I：

10.13220/j.cnki.jipr.2010.04.001

中图分类号：

Q503 [生物化学技术];

学科分类号：

071010 ; 081704 ;

摘要：

指数富集的配体系统进化(SELEX)技术是一类具备蓬勃发展前景的体外筛选技术,在生物学、药学及化学领域已引起广泛关注。本文即针对2004年以来SELEX技术的发展特点,主要介绍两类新型SELEX策略,即毛细管电泳-SELEX和针对复杂靶标的SELEX方法,并简要总结了寡核苷酸适配体在生物医学和药学相关方面的最新应用进展。

引用

页码：249 / 256

页数：8

共 13 条

[1]

基于毛细管电泳的细胞SELEX方法的初步建立.[J].徐华;李少华;郭磊;张朝阳;谢剑炜;邵宁生;.军事医学科学院院刊.2009, 05

[2] Selection of aptamers for signal transduction proteins by capillary electrophoresis [J].

Tok, Junie ;

Lai, Jesyin ;

Leung, Thomas ;

Li, Sam Fong Yau .

ELECTROPHORESIS, 2010, 31 (12) :2055-2062

[3] Generating Aptamers for Recognition of Virus-Infected Cells [J].

Tang, Zhiwen ;

Parekh, Parag ;

Turner, Pete ;

Moyer, Richard W. ;

Tan, Weihong .

CLINICAL CHEMISTRY, 2009, 55 (04) :813-822

[4] Phase 1b randomized study of antidote-controlled modulation of factor IXa activity in patients with stable coronary artery disease [J].

Chan, Mark Y. ;

Cohen, Mauricio G. ;

Dyke, Christopher K. ;

Myles, Shelley K. ;

Aberle, Laura G. ;

Lin, Min ;

Walder, James ;

Steinhubl, Steven R. ;

Gilchrist, Ian C. ;

Kleiman, Neal S. ;

Vorchheimer, David A. ;

Chronos, Nicholas ;

Melloni, Chiara ;

Alexander, John H. ;

Harrington, Robert A. ;

Tonkens, Ross M. ;

Becker, Richard C. ;

Rusconi, Christopher P. .

CIRCULATION, 2008, 117 (22) :2865-2874

[5] Aptamer from whole-bacterium SELEX as new therapeutic reagent against virulent Mycobacterium tuberculosis [J].

Chen, Fan ;

Zhou, Jing ;

Luo, Fengling ;

Mohammed, Al-Bayati ;

Zhang, Xiao-Lian .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 357 (03) :743-748

[6] First-in-human experience of an antidote-controlled anticoagulant using RNA aptamer technology - A phase 1a pharmacodynamic evaluation of a drug-antidote pair for the controlled regulation of factor IXa activity [J].

Dyke, Christopher K. ;

Steinhubl, Steven R. ;

Kleiman, Neal S. ;

Cannon, Richard O. ;

Aberle, Laura G. ;

Lin, Min ;

Myles, Shelley K. ;

Melloni, Chiara ;

Harrington, Robert A. ;

Alexander, John H. ;

Becker, Richard C. ;

Rusconi, Christopher P. .

CIRCULATION, 2006, 114 (23) :2490-2497

[7]

An Aptamer–Doxorubicin Physical Conjugate as a Novel Targeted Drug‐Delivery Platform.[J].Vaishali Bagalkot;Omid C. Farokhzad Prof. Dr.;Robert Langer Prof. Dr.;Sangyong Jon Prof. Dr..Angewandte Chemie.2006, 48

[8] Selection of RNA aptamers against recombinant transforming growth factor-β type III receptor displayed on cell surface [J].

Ohuchi, Shoji P. ;

Ohtsu, Takashi ;

Nakamura, Yoshikazu .

BIOCHIMIE, 2006, 88 (07) :897-904

[9]

A screening method for DNA aptamers that bind to?a?specific; unidentified protein in tissue samples.[J].Takahisa Noma;Kazunori Ikebukuro;Koji Sode;Takuya Ohkubo;Yuji Sakasegawa;Naomi Hachiya;Kiyotoshi Kaneko.Biotechnology Letters.2006, 17

[10] Pharmacologic and pharmacokinetic assessment of anti-TGFβ2 aptamers in rabbit plasma and aqueous humor [J].

McCauley, TG ;

Kurz, JC ;

Merlino, PG ;

Lewis, SD ;

Gilbert, M ;

Epstein, DM ;

Marsh, HN .

PHARMACEUTICAL RESEARCH, 2006, 23 (02) :303-311

← 1 2 →