Selection of RNA aptamers against recombinant transforming growth factor-β type III receptor displayed on cell surface

被引：118

作者：

Ohuchi, Shoji P. ^{[1
]}

Ohtsu, Takashi ^{[1
]}

Nakamura, Yoshikazu ^{[1
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Basic Med Sci, Minato Ku, Tokyo 1088639, Japan

来源：

BIOCHIMIE | 2006年 / 88卷 / 07期

关键词：

RNA aptamer; TECS-SELEX; TGF-beta type III receptor; cell surface receptors;

D O I：

10.1016/j.biochi.2006.02.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In most cases, anti-protein aptamers are selected by systematic evolution of ligands by exponential enrichment (SELEX) using purified recombinant protein targets. Cell surface proteins, however, are not easy targets for SELEX due to the difficulties associated with their purification. Here, we developed a novel SELEX procedure (referred to as TECS-SELEX) in which cell-surface displayed recombinant protein is directly used as the selection target. Using this method, we isolated RNA aptamers against transforming growth factor-beta type III receptor expressed on Chinese hamster ovary (CHO) cells. One of the RNA aptamers has a dissociation constant in the 1 nM range and competed with transforming growth factor-P to bind to the cell surface receptor in vitro. (c) 2006 Elsevier SAS. All rights reserved.

引用

页码：897 / 904

页数：8

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