金黄色葡萄球菌LAMP可视化快速检测方法的建立

被引:9
作者
宋涛平
邱华丽
王淑娟
彭新凯
杨丽霞
机构
[1] 长沙市食品质量安全监督检测中心
关键词
金黄色葡萄球菌; 环介导等温扩增; 可视化检测;
D O I
10.13652/j.issn.1003-5788.2015.05.013
中图分类号
TS207.4 [食品的微生物检验];
学科分类号
摘要
建立金黄色葡萄球菌LAMP可视化检测方法。通过对2套引物的筛选,最终选取nuc基因的6条引物建立LAMP反应体系。整个反应在63℃恒温条件下进行45min,即可通过肉眼直接对反应结果进行判定。对金黄色葡萄球菌的检测灵敏度为0.001ng/μL,且与其他致病菌无交叉反应。该方法快速、特异性高,操作简便,适合基层快速检测。
引用
收藏
页码:55 / 58
页数:4
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