神经干细胞移植促进大鼠脊髓损伤后前角运动神经元存活及后肢运动功能恢复的实验研究

被引：12

作者：

孔令胜

聂冬丽

张军臣

张浩

许华

机构：

[1] 济宁医学院附属医院神经外科

来源：

实用医学杂志 | 2009年 / 25卷 / 20期

关键词：

脊髓损伤; 干细胞移植; 前角运动神经元; 后肢功能;

D O I：

暂无

中图分类号：

R651.2 [脊髓];

学科分类号：

摘要：

目的:研究神经干细胞(NSCs)移植对大鼠脊髓损伤(SCI)后前角神经元的作用及后肢运动功能的恢复。方法:5-溴脱氧尿嘧啶核苷(BrdU)法标记处于对数生长期的NSCs,采用电控脊髓损伤打击装置制作大鼠SCI模型。实验分为BrdU+NSCs组、NSCs组、SCI组、假手术组(Sham组)4组。SCI后3d进行NSCs移植,应用免疫组化法观察BrdU标记的移植细胞的存活及迁移情况,Nissl染色法观察脊髓前角运动神经元存活情况,采用行为学(BBB)评分法观察大鼠后肢运动功能的恢复情况。结果:BrdU+NSCs组在损伤脊髓区域可检测到BrdU标记的阳性NSCs,BrdU+NSCs组和NSCs组脊髓前角存活运动神经元较SCI组明显增多(P<0.05),BBB评分亦明显高于SCI组(均P<0.05)。结论:体外培养的胚胎大鼠NSCs在移植到SCI区域后可存活、迁移,对SCI后前角运动神经元具有保护作用,从而促进了大鼠后肢运动功能的恢复。

引用

页码：3402 / 3404

页数：3

共 7 条

[1] 胚胎大鼠神经干细胞的分离培养及鉴定研究 [J].

孔令胜 ;

张军臣 ;

张冉 ;

赵万巨 ;

邵彤 ;

杨全祥 .

济宁医学院学报, 2009, 32 (02) :96-99

[2] Functional outcome of bone marrow stem cells (CD45+/CD34-) after cell therapy in chronic spinal cord injury in Wistar rats [J].

Carvalho, K. A. T. ;

Vialle, E. N. ;

Moreira, G. H. G. ;

Cunha, R. C. ;

Simeoni, R. B. ;

Francisco, J. C. ;

Guarita-Souza, L. C. ;

Oliveira, L. ;

Zocche, L. ;

Olandoski, M. .

TRANSPLANTATION PROCEEDINGS, 2008, 40 (03) :845-846

[3]

Treatment of chronic thoracic spinal cord injury patients with autologous Schwann cell transplantation: An interim report on safety considerations and possible outcomes[J] . Hooshang Saberi,Pouria Moshayedi,Hamid-Reza Aghayan,Babak Arjmand,Seyed-Kazem Hosseini,Seyed-Hassan Emami-Razavi,Vafa Rahimi-Movaghar,Mohsin Raza,Masoumeh Firouzi.Neuroscience Letters . 2008 (1)

[4] Migrating and myelinating potential of subventricular zone neural progenitor cells in white matter tracts of the adult rodent brain [J].

Cayre, M ;

Bancila, M ;

Virard, I ;

Borges, A ;

Durbec, P .

MOLECULAR AND CELLULAR NEUROSCIENCE, 2006, 31 (04) :748-758

[5]

Neural stem cells may be uniquely suited for combined gene therapy and cell replacement: Evidence from engraftment of Neurotrophin-3-expressing stem cells in hypoxic–ischemic brain injury[J] . Kook In Park,B. Timothy Himes,Philip E. Stieg,Alan Tessler,Itzhak Fischer,Evan Y. Snyder.Experimental Neurology . 2006 (1)

[6] Neural stem cells constitutively secrete neurotrophic factors and promote extensive host axonal growth after spinal cord injury [J].

Lu, P ;

Jones, LL ;

Snyder, EY ;

Tuszynski, MH .

EXPERIMENTAL NEUROLOGY, 2003, 181 (02) :115-129

[7] Human neural precursor cells express low levels of telomerase in vitro and show diminishing cell proliferation with extensive axonal outgrowth following transplantation [J].

Ostenfeld, T ;

Caldwell, MA ;

Prowse, KR ;

Linskens, MH ;

Jauniaux, E ;

Svendsen, CN .

EXPERIMENTAL NEUROLOGY, 2000, 164 (01) :215-226

← 1 →