高效液相色谱法同时检测粮食中常见8种真菌毒素的含量

被引：53

作者：

黎睿

谢刚

王松雪

机构：

[1] 不详

[2] 国家粮食局科学研究院

[3] 不详

来源：

食品科学 | 2015年 / 06期

基金：

国家高技术研究发展计划(863计划);

关键词：

粮食; 黄曲霉毒素B1、B2、G1、G2; 玉米赤霉烯酮; 脱氧雪腐镰刀菌烯醇; T-2毒素; 赭曲霉毒素A; 高效液相色谱; 免疫亲和柱;

D O I：

暂无

中图分类号：

TS210.7 [产品标准与检验]; O657.72 [];

学科分类号：

摘要：

建立免疫亲和柱净化-高效液相色谱法同时测定粮食中黄曲霉毒素B1（aflatoxins,AFB1）、黄曲霉毒素B2（AFB2）、黄曲霉毒素G1（AFG1）、黄曲霉毒素G2（AFG2）、赭曲霉毒素A（ochratoxin A,OTA）、玉米赤霉烯酮（zearalenone,ZEN）、呕吐毒素（deoxynivalenol,DON）和T-2毒素的检测方法。样品经乙腈-水提取后,用免疫亲和柱净化,Agilent Elipse Plus C18（100 mm×4 mm,3.5μm）色谱柱分离,以甲醇-乙腈-水-乙酸为流动相,流速1 m L/min,柱温35℃,进样量20μL,检测系统为可变波长检测器串联光化学衍生器串联荧光检测器。根据信噪比为3的峰响应值,确定各真菌毒素的检出限为:AFB1 0.446 ng/m L、AFB2 0.152 ng/m L、AFG1 0.523 ng/m L、AFG2 0.334 ng/m L、ZEN 7 ng/m L、OTA 0.7 ng/m L、DON 200 ng/m L、T-2毒素100 ng/m L。样品中各真菌毒素的平均加标回收率,玉米为80.0%104.5%,小麦为83.2%102.8%,方法精密度为2.6%10.2%。从样品前处理到分析整个过程耗时约2 h。本方法简便、快速、灵敏度高,适用于粮食中多种真菌毒素的快速测定。

引用

页码：206 / 210

页数：5

共 17 条

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