阿霉素与胃癌单克隆抗体交联物的体内外抗肿瘤作用

被引：4

作者：

张运涛

王耐勤

李农

刘彤

董志伟

机构：

[1] 北京市肿瘤防治研究所,北京市肿瘤防治研究所,北京市肿瘤防治研究所,北京市肿瘤防治研究所,北京市肿瘤防治研究所北京,北京,北京,北京,北京

来源：

药学学报 | 1992年 / 05期

关键词：

单克隆抗体; 阿霉素; 丝裂霉素C; 免疫交联物; 抗肿瘤作用;

D O I：

10.16438/j.0513-4870.1992.05.002

中图分类号：

学科分类号：

摘要：

以氧化葡聚糖法制备了阿霉素（ADM）与胃癌单克隆抗体（MoAb）3H11的交联物3H11-DEX-ADM,ADM与3H11克分子比为73:1。经ELISA法测定,交联物的抗体活性保留86％。体外细胞毒试验显示,3H11-DEX-ADM对胃癌细胞BGC823的杀伤作用比游离ADM明显增强,其IC50分别为1.0μg/ml及3.75μg/ml。在荷瘤裸鼠治疗实验中3H11-DEX-ADM能显著抑制肿瘤生长,抑制率为51.5％（P<0.05）,明显高于ADM组及其非特异性抗体交联物（NI gG-DEX-ADM）组,后者的抑制率分别为21％及24％。表明3H11-DEX-ADM对肿瘤具有选择杀伤作用。将3H11-DEX-ADM与丝裂霉素C（MMC）-3H11交联物（3H11-HSA-MMC）联合应用,细胞素实验未能显示协同或相加作用;游离ADM与MMC联合亦呈相似结果。

引用

页码：325 / 330

页数：6

共 6 条

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