重组乙酰胆碱酯酶的表达及其分子进化研究进展

被引:4
作者
董洁娴
李振峰
雷红涛
何永盛
王弘
孙远明
机构
[1] 华南农业大学食品学院广东省食品质量安全重点实验室
基金
广东省科技计划;
关键词
农药残留; 重组乙酰胆碱酯酶; 酶抑制检测法;
D O I
10.13345/j.cjb.2012.05.002
中图分类号
S481.8 []; Q78 [基因工程(遗传工程)];
学科分类号
071007 ; 0836 ; 090102 ;
摘要
乙酰胆碱酯酶(Acetylcholinesterase,AChE)是酶抑制法检测农药残留的核心试剂。但天然提取的AChE存在含量低、纯化困难、稳定性差等不足,因此,高性能的重组AChE制备成为研究学者关注的热点。文中综述了重组AChE的表达、和分子进化等方面的研究成果,并指出酶定向进化策略与表面展示技术结合是重组AChE活性改造的未来趋势。
引用
收藏
页码:557 / 564
页数:8
相关论文
共 18 条
  • [1] Cloning and Expression Analysis of Acetylcholinesterase Gene (Bm-ace1, Bm-ace2) from Domesticated Silkworm, Bombyx mori[J] . Li Bing,Wang Yan Hong,Wang Ju Mei,Shen Wei De.Advanced Materials Research . 2011 (175)
  • [2] Enantioselective Interaction with Acetylcholinesterase of an Organophosphate Insecticide Fenamiphos
    Wang, Cui
    Zhang, Na
    Li, Ling
    Zhang, Quan
    Zhao, Meirong
    Liu, Weiping
    [J]. CHIRALITY, 2010, 22 (06) : 612 - 617
  • [3] Heterologous expression, purification, and biochemical characterization of a greenbug (Schizaphis graminum) acetylcholinesterase encoded by a paralogous gene (ace‐1)[J] . PichengZhao,Kun YanZhu,HaoboJiang.J. Biochem. Mol. Toxicol. . 2010 (1)
  • [4] Expression of the housefly acetylcholinesterase in a bioreactor and its potential application in the detection of pesticide residues[J] . Guo-Jun Lang,Jin-Yan Shang,Yan-Xia Chen,Ying-Jun Cui,Qiang Wang,Zhen-Hua Tang,Chuan-Xi Zhang.World Journal of Microbiology and Biotechnology . 2010 (10)
  • [5] Recombinant expression and biochemical characterization of the catalytic domain of acetylcholinesterase-1 from the African malaria mosquito, Anopheles gambiae[J] . Haobo Jiang,Siwei Liu,Picheng Zhao,Carey Pope.Insect Biochemistry and Molecular Biology . 2009 (9)
  • [6] The use of Artificial Neural Networks for the selective detection of two organophosphate insecticides: Chlorpyrifos and chlorfenvinfos[J] . Georges Istamboulie,Montserrat Cortina-Puig,Jean-Louis Marty,Thierry Noguer.Talanta . 2009 (2)
  • [7] Molecular cloning of acetylcholinesterase gene from Salicornia europaea L
    Yamamoto, Kosuke
    Oguri, Suguru
    Chiba, Susumu
    Momonoki, Yoshie S.
    [J]. PLANT SIGNALING & BEHAVIOR, 2009, 4 (05) : 361 - 366
  • [8] High yield production of a mutant Nippostrongylus brasiliensis acetylcholinesterase in Pichia pastoris and its purification
    Richter, S
    Nieveler, J
    Schulze, H
    Bachmann, TT
    Schmid, RD
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 2006, 93 (05) : 1017 - 1022
  • [9] High-level secretion and purification of recombinant acetylcholinesterase from human cerebral tissue in P. pastoris and identification by chromogenic reaction[J] . Xingyuan Ma,Jianhua Tan,Dongzhi Wei,Pin Zhu,Manji Sun.Applied Microbiology and Biotechnology . 2006 (2)
  • [10] Purification of transgenic plant-derived recombinant human acetylcholinesterase-R[J] . Brian C. Geyer,Mrinalini Muralidharan,Irene Cherni,Jeffrey Doran,Samuel P. Fletcher,Tama Evron,Hermona Soreq,Tsafrir S. Mor.Chemico-Biological Interactions . 2005