重组乙酰胆碱酯酶的表达及其分子进化研究进展

被引：4

作者：

董洁娴

李振峰

雷红涛

何永盛

王弘

孙远明

机构：

[1] 华南农业大学食品学院广东省食品质量安全重点实验室

来源：

生物工程学报 | 2012年 / 05期

基金：

广东省科技计划;

关键词：

农药残留; 重组乙酰胆碱酯酶; 酶抑制检测法;

D O I：

10.13345/j.cjb.2012.05.002

中图分类号：

S481.8 []; Q78 [基因工程（遗传工程）];

学科分类号：

071007 ; 0836 ; 090102 ;

摘要：

乙酰胆碱酯酶(Acetylcholinesterase,AChE)是酶抑制法检测农药残留的核心试剂。但天然提取的AChE存在含量低、纯化困难、稳定性差等不足,因此,高性能的重组AChE制备成为研究学者关注的热点。文中综述了重组AChE的表达、和分子进化等方面的研究成果,并指出酶定向进化策略与表面展示技术结合是重组AChE活性改造的未来趋势。

引用

页码：557 / 564

页数：8

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