生长素和细胞分裂素对平邑甜茶组培苗PAs和NO含量的影响

被引:17
作者
张龙
杨洪强
李强
孙晓莉
乔海涛
机构
[1] 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室
关键词
平邑甜茶; 一氧化氮; 多胺; 生长素; 细胞分裂素;
D O I
10.16420/j.issn.0513-353x.2009.07.005
中图分类号
S661.1 [苹果];
学科分类号
090201 [果树学];
摘要
以平邑甜茶继代组培苗为试材,研究了NAA、IBA、ZT和6-BA作用下组培苗的生长、内源多胺(PAs)和一氧化氮(NO)含量的变化。结果表明,在MS培养基中添加0.02~1.00mg.L-1的NAA或IBA后,外植体鲜样质量增加率和蛋白质含量以及多胺含量和一氧化氮含量均明显增加,其中浓度在0.10~0.50mg.L-1时增加最高;同样浓度下,NAA处理的外植体鲜样质量增加率和腐胺含量高于IBA。在0.08~4.00mg.L-1范围内,随着ZT或6-BA浓度的升高,外植体鲜样质量增加率、可溶性蛋白含量、多胺含量和一氧化氮含量均逐渐升高;同样浓度下,6-BA处理的外植体鲜样质量增率和腐胺含量高于ZT。生长素和细胞分裂素促进组培苗生长与促进内源PAs和NO的生成相伴随,组培苗生长动态与内源多胺和一氧化氮含量的变化趋势一致。
引用
收藏
页码:953 / 958
页数:6
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