Zip1转染骨髓间充质干细胞对Wnt1/β-catenin信号成骨通路的影响

被引：3

作者：

章晓云 ^{[1
]}

夏天 ^{[2
]}

陈跃平 ^{[1
]}

廖小林 ^{[2
]}

袁振中 ^{[2
]}

卓映宏 ^{[2
]}

冯洋 ^{[2
]}

蓝佼 ^{[2
]}

赵斌 ^{[2
]}

机构：

[1] 广西中医药大学附属瑞康医院创伤骨科与手外科

[2] 广西中医药大学

来源：

中国骨质疏松杂志 | 2018年 / 24卷 / 05期

关键词：

骨髓间充质干细胞; 锌转运蛋白1; 碱性磷酸酶; 骨钙素; 成骨分化;

D O I：

暂无

中图分类号：

R329.2 [人体细胞学];

学科分类号：

100107 [人体解剖与组织胚胎学（人体解剖学、组织与胚胎学）];

摘要：

目的观察ZIP1 siRNA转染的骨髓间充质干细胞对Wnt1/β-catenin信号成骨通路的影响。方法从成年雄性新西兰兔股骨骨髓中提取骨髓间充质干细胞并培养传代,然后使用0.03 mol/L,0.09 mol/L,0.15 mol/L,0.21 mol/L酒精进行培养,经ZIP1 siRNA及ZIP1表达转染后,检测各组骨髓间充质干细胞中ALP、I型胶原、OCN及Runx2蛋白的表达量。结果与空白对照组相比,不同酒精组的ALP、I型胶原、OCN及Runx2蛋白表达量均有所下降,随着浓度升高,其表达量逐渐下降,但0.03 mol/L组中ALP及OCN下降不明显,统计学无明显意义(P>0.05),其他各组均有明显的统计学意义(P<0.05),不同酒精组中的I型胶原及Runx2与正常组之间差异均有明显统计学意义(P<0.05)。与空白对照组对比,ZIP1表达组ALP、I型胶原及OCN表达量明显升高,差异具有明显的统计学意义(P<0.05),ZIP1siRNA组ALP、OCN表达量明显降低,差异具有明显的统计学意义(P<0.05),而I型胶原表达量下降不明显,差异无统计学意义(P>0.05);与ZIP1siRNA组相比,ZIP1表达组ALP、I型胶原及OCN表达量明显升高,差异有统计学意义(P<0.05)。结论 ZIP1能促使骨髓间质干细胞向成骨分化,从而促进骨修复及骨代谢。沉默ZIP1表达能抑制骨髓间充质干细胞的WNT1/β-catenin信号成骨通路的表达,从而抑制促进细胞的成骨分化能力。

引用

页码：594 / 599

页数：6

共 16 条

[1]

Expression of the ZIP/ SLC39A transporters in β-cells: a systematic review and integration of multiple datasets.[J].Rebecca Lawson;Wolfgang Maret;Christer Hogstrand.BMC Genomics.2017, 1

[2]

Berberine promotes bone marrow-derived mesenchymal stem cells osteogenic differentiation via canonical Wnt/β-catenin signaling pathway [J].

Tao Ke ;

Xiao Deming ;

Weng Jian ;

Xiong Ao ;

Kang Bin ;

Zeng Hui .

TOXICOLOGY LETTERS, 2016, 240 (01) :68-80

[3]

Activation of zinc-requiring ectoenzymes by ZnT transporters during the secretory process: Biochemical and molecular aspects.[J].Taiho Kambe;Taka-aki Takeda;Yukina Nishito.Archives of Biochemistry and Biophysics.2016,

[4]

THE PHYSIOLOGICAL, BIOCHEMICAL, AND MOLECULAR ROLES OF ZINC TRANSPORTERS IN ZINC HOMEOSTASIS AND METABOLISM [J].

Kambe, Taiho ;

Tsuji, Tokuji ;

Hashimoto, Ayako ;

Itsumura, Naoya .

PHYSIOLOGICAL REVIEWS, 2015, 95 (03) :749-784

[5]

Zinc: An antioxidant and anti-inflammatory agent: Role of zinc in degenerative disorders of aging [J].

Prasad, Ananda S. .

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, 2014, 28 (04) :364-371

[6]

Current understanding of ZIP and ZnT zinc transporters in human health and diseases [J].

Kambe, Taiho ;

Hashimoto, Ayako ;

Fujimoto, Shigeyuki .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2014, 71 (17) :3281-3295

[7]

ZIP1 and Zinc Inhibits Fluoride-Induced Apoptosis in MC3T3-E1 Cells [J].

Xu, Shihong ;

Yang, Yongliang ;

Han, Shumei ;

Wu, Zonghui .

BIOLOGICAL TRACE ELEMENT RESEARCH, 2014, 159 (1-3) :399-409

[8]

Myricetin enhances osteogenic differentiation through the activation of canonical Wnt/β-catenin signaling in human bone marrow stromal cells.[J].Xiaozhou Ying;Xiaowei Chen;Yongzeng Feng;Hua zi Xu;Hua Chen;Kehe Yu;Shaowen Cheng;Lei Peng.European Journal of Pharmacology.2014,

[9]

TPEN Induces Apoptosis Independently of Zinc Chelator Activity in a Model of Acute Lymphoblastic Leukemia and Ex Vivo Acute Leukemia Cells through Oxidative Stress and Mitochondria Caspase-3- and AIF-Dependent Pathways.[J].Miguel Mendivil-Perez;Carlos Velez-Pardo;Marlene Jimenez-Del-Rio;Felipe Dal-Pizzol.Oxidative Medicine and Cellular Longevity.2012,

[10]

Zinc supplementation prevents cardiomyocyte apoptosis and congenital heart defects in embryos of diabetic mice [J].

Kumar, Srinivasan Dinesh ;

Vijaya, Murugaiyan ;

Samy, Ramar Perumal ;

Dheen, S. Thameem ;

Ren, Minqin ;

Watt, Frank ;

Kang, Y. James ;

Bay, Boon-Huat ;

Tay, Samuel Sam Wah .

FREE RADICAL BIOLOGY AND MEDICINE, 2012, 53 (08) :1595-1606

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