Gαi2 regulates the adult myogenesis of masticatory muscle satellite cells

被引：4

作者：

Kong, Lin ^{[1
,2
]}

Fang, Yi ^{[1
,2
,3
,4
]}

Du, Mingyuan ^{[1
,2
,5
]}

Wang, Yunlong ^{[1
,2
,5
]}

He, Hong ^{[1
,2
,5
]}

Liu, Zhijian ^{[1
,2
,5
,6
]}

机构：

[1] Wuhan Univ, State Key Lab Breeding Base Basic Sci Stomatol Hub, Wuhan, Peoples R China

[2] Wuhan Univ, Sch & Hosp Stomatol, Key Lab Oral Biomed, Minist Educ, Wuhan, Peoples R China

[3] Kunming Med Univ, Sch & Hosp Stomatol, Kunming, Peoples R China

[4] Yunnan Key Lab Stomatol, Kunming, Peoples R China

[5] Wuhan Univ, Sch & Hosp Stomatol, Dept Orthodont, Wuhan, Peoples R China

[6] Wuhan Univ, Sch & Hosp Stomatol, Dept Orthodont, Luoyu Rd 237, Wuhan 430079, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2023年 / 27卷 / 09期

基金：

中国国家自然科学基金;

关键词：

adult myogenesis; G alpha i2; masticatory muscle; muscle satellite cell; SKELETAL-MUSCLE; EXPRESSION; ACTIVATION; HEAD;

D O I：

10.1111/jcmm.17726

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Although similar to trunk and limb skeletal muscles, masticatory muscles are believed as unique in both developmental origins and myogenesis. Gai2 has been demonstrated to promote muscle hypertrophy and muscle satellite cell differentiation in limb muscles. However, the effect of Gai2 on masticatory muscles is still unexplored. This study aimed to identify the role of Gai2 in the proliferation and differentiation of masticatory muscle satellite cells, further exploring the metabolic mechanism of masticatory muscles. The proliferation rate, myotube size, fusion index of masticatory muscle satellite cells and Pax7, Myf5, MyoD, Tcf21 and Musculin expressions were significantly decreased by Gai2 knockdown, while in cells infected with AdV4-Gai2, the proliferation rate, myotube size, fusion index and Tbx1 expression were significantly increased. Masticatory muscle satellite cells also displayed phenotype transformation as Gai2 changed. In addition, Gai2 altered myosin heavy chain (MyHC) isoforms of myotubes with less MyHC-2A expression in siGai2 group and more MyHC-slow expression in AdV4-Gai2 group. In conclusion, Gai2 could positively affect the adult myogenesis of masticatory muscle satellite cells and maintain the superiority of MyHC-slow. Masticatory muscle satellite cells may have their unique Gai2-regulated myogenic transcriptional networks, although they may share some common characteristics with trunk and limb muscles.

引用

页码：1239 / 1249

页数：11

共 45 条

[21]

Pax7 activates myogenic genes by recruitment of a histone methyltransferase complex [J].