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Urinary high performance reverse phase chromatography cortisol and cortisone analyses before and at the end of a race in elite cyclists

被引：47
作者：
Gatti, R
Cappellin, E
Zecchin, B
Antonelli, G
Spinella, P
Mantero, F
De Palo, EF
机构：
[1] Univ Padua, Clin Biochem Sect, Dept Med Diagnost Sci & Spec Ther, I-35100 Padua, Italy
[2] Univ Padua, Dept Surg & Med Sci, UO Endocrinol, I-35100 Padua, Italy
[3] ULSS 15, Div Geriatr, Camposampiero, PD, Italy
[4] Univ Padua, Dept Clin & Expt Med, I-35100 Padua, Italy
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 824卷 / 1-2期
关键词：
solid phase extraction; exercise; 11; beta-HSD;
D O I：
10.1016/j.jchromb.2005.06.037
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A functional and basic method for the quantitative analysis of urine cortisol (F) and cortisone (E) using a Solid-Phase Extraction column and HPLC with ultraviolet detection is here described and validated to analyse urine samples. Urine specimens were analysed to study F and E relation and ratio in athletes and healthy sedentary subjects. The F and E concentrations in random urine specimens were significantly higher in the post exercise versus pre exercise condition in cyclists (F: 136 +/- 93 nmol/l versus 67 +/- 50 nmol/l (p < 0.001); E: 797 +/- 400 nmol/l versus 408 +/- 252 nmol/l (p < 0.001)). The F/E ratio was 0.18 +/- 0.11 versus 0.16 +/- 0.07, respectively, and a significant difference was only demonstrated comparing sedentary (0.11 +/- 0.07) and cyclist individuals at rest (P < 0.05). (c) 2005 Elsevier B.V. All rights reserved.
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收藏
页码：51 / 56
页数：6
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