Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes -: Part 2.: Bienzymatic configuration

被引：9

作者：

Cortón, E

Battaglini, F

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, INQUIMAE, Dept Quim Inorgan Analit & Quim Fis, RA-1428 Buenos Aires, DF, Argentina

[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Biol, RA-1428 Buenos Aires, DF, Argentina

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2001年 / 511卷 / 1-2期

关键词：

biosensors; chronoamperometry; cyclic voltammetry; flow injection analysis; redox enzymes;

D O I：

10.1016/S0022-0728(01)00500-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Four configurations of an enzyme electrode to assess lactate concentration in dairy products are studied. In these bienzymatic multilayer systems, the inner layer was constructed by crosslinking peroxidase with poly(allylamine) containing an osmium complex (OsPAA), and an outer layer containing lactate oxidase (LOx) immobilized in polymeric matrices. When the hydrogen peroxide formed by the LOx layer reaches the inner layer, the electronic flow between the wired peroxidase and the electrode surface produces a cathodic current, proportional to lactate concentration. Parameters such as the effect of ionic strength, stability when the electrode is used as detector in a FIA system and reproducibility were examined. This arrangement allows working in undiluted milk samples. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：8 / 12

页数：5

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