Biosensing with conically shaped nanopores and nanotubes

被引：89

作者：

Choi, Youngseon ^{[1
]}

Baker, Lane A. ^{[1
]}

Hillebrenner, Heather ^{[1
]}

Martin, Charles R. ^{[1
]}

机构：

[1] Univ Florida, Res Ctr, Dept Chem, Bionano Interface, Gainesville, FL 32611 USA

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2006年 / 8卷 / 43期

关键词：

D O I：

10.1039/b607360c

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

In this review we consider recent results from our group that are directed towards developing "smart'' synthetic nanopores that can mimic the functions of biological nanopores ( transmembrane proteins). We first discuss the preparation and characterization of conical nanopores synthesized using the track-etch process. We then consider the design and function of conical nanopores that can rectify the ionic current that flows through these pores under an applied transmembrane potential. Finally, two types of sensors that we have developed with these conical nanopores are described. The first sensor makes use of molecular recognition elements that are bound to the nanopore mouth to selectively block the nanopore tip, thus detecting the presence of the analyte. The second sensor makes use of conical nanopores in a resistive-pulse type experiment, detecting the analyte via transient blockages in ionic current.

引用

页码：4976 / 4988

页数：13

共 87 条

[1]

Alberts B., 2002, Molecular Biology of The Cell, V4th

[2] Diode-like single-ion track membrane prepared by electro-stopping [J].

Apel, PY ;

Korchev, YE ;

Siwy, Z ;

Spohr, R ;

Yoshida, M .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 2001, 184 (03) :337-346

[3] Toward single molecule DNA sequencing:: Direct identification of ribonucleoside and deoxyribonucleoside 5′-monophosphates by using an engineered protein nanopore equipped with a molecular adapter [J].

Astier, Y ;

Braha, O ;

Bayley, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (05) :1705-1710

[4] Biomaterials and biotechnologies based on nanotube membranes [J].

Baker, LA ;

Jin, P ;

Martin, CR .

CRITICAL REVIEWS IN SOLID STATE AND MATERIALS SCIENCES, 2005, 30 (04) :183-205

[5]

Bard A. J., 2001, Electrochem. Methods Fundam. Appl, V2nd

[6] Resistive-pulse sensing - From microbes to molecules [J].

Bayley, H ;

Martin, CR .

CHEMICAL REVIEWS, 2000, 100 (07) :2575-2594

[7] Stochastic sensors inspired by biology [J].

Bayley, H ;

Cremer, PS .

NATURE, 2001, 413 (6852) :226-230

[8] Functional engineered channels and pores - (Review) [J].

Bayley, H ;

Jayasinghe, L .

MOLECULAR MEMBRANE BIOLOGY, 2004, 21 (04) :209-220

[9] Field-dependent effect of crown ether (18-crown-6) on ionic conductance of α-hemolysin channels [J].

Bezrukov, SM ;

Krasilnikov, OV ;

Yuldasheva, LN ;

Berezhkovskii, AM ;

Rodrigues, CG .

BIOPHYSICAL JOURNAL, 2004, 87 (05) :3162-3171

[10] The charge state of an ion channel controls neutral polymer entry into its pore [J].

Bezrukov, SM ;

Kasianowicz, JJ .

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1997, 26 (06) :471-476

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