Penicillin detection with nanocrystalline-diamond field-effect sensor

被引：25

作者：

Abouzar, Maryam H. ^{[1
,2
]}

Poghossian, Arshak ^{[1
,2
]}

Razavi, Arash ^{[1
,2
]}

Besmehn, Astrid ^{[5
]}

Bijnens, Nathalile ^{[3
]}

Williams, Oliver A. ^{[3
,4
]}

Haenen, Ken ^{[3
,4
]}

Wagner, Patrick ^{[3
]}

Schoening, Michael J. ^{[1
,2
]}

机构：

[1] Aachen Univ Appl Sci, INB, D-52428 Julich, Germany

[2] Res Ctr Julich GmbH, Inst Bio & Nanosyst IBN 2, D-52425 Julich, Germany

[3] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium

[4] IMEC VZW, Div IMOMEC, B-3590 Diepenbeek, Belgium

[5] Res Ctr Julich GmbH, Cent Div Analyt Chem ZCH, D-52425 Julich, Germany

来源：

PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE | 2008年 / 205卷 / 09期

关键词：

D O I：

10.1002/pssa.200879713

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Nanocrystalline-diamond (NCD) films have been utilised for the detection of penicillin G for the first time. The developed penicillin-sensitive biosensor consists of a field-effect capacitive electrolyte-diamond-insulator-semiconductor (EDIS) structure with an immobilised enzyme layer that covers the gate region of the sensor. Undoped NCD thin films of about 100 nm thickness were grown on a p-Si-SiO2 (50 nm thermally grown SiO2) structure by microwave plasma-enhanced chemical vapour deposition. The enzyme penicillinase has been adsorptively immobilised directly onto the O-terminated NCD surface. The EDIS biosensors have been characterised in buffer solutions with different content of penicillin G by means of capacitance-voltage and constant-capacitance method. The developed penicillin biosensor possesses a low detection limit of 5 mu M and a high sensitivity of 6070 mV/decade in a wide linear range of 0.005-2.5 mM penicillin G concentration. (C) 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：2141 / 2145

页数：5

共 25 条

[1]

Arakawa Hiroshi, 2001, BMC Biotechnology, V1, P1

[2] pH sensitivity of nanocrystalline diamond films [J].

Christiaens, P. ;

Abouzar, M. H. ;

Poghossian, A. ;

Wagner, T. ;

Bijnens, N. ;

Williams, O. A. ;

Daenen, M. ;

Haenen, K. ;

Douheret, O. ;

D'Haen, J. ;

Mekhalif, Z. ;

Schoening, M. J. ;

Wagner, P. .

PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 2007, 204 (09) :2925-2930

[3] Seeding, growth and characterization of nanocrystalline diamond films on various substrates [J].

Daenen, M. ;

Williams, O. A. ;

D'Haen, J. ;

Haenen, K. ;

Nesladek, M. .

PHYSICA STATUS SOLIDI A-APPLICATIONS AND MATERIALS SCIENCE, 2006, 203 (12) :3005-3010

[4] Resolving the controversy on the pH sensitivity of diamond surfaces [J].

Dankerl, M. ;

Reitinger, A. ;

Stutzmann, M. ;

Garrido, J. A. .

PHYSICA STATUS SOLIDI-RAPID RESEARCH LETTERS, 2008, 2 (01) :31-33

[5] pH sensor on O-terminated diamond using boron-doped channel [J].

Denisenko, A. ;

Jamornmarn, G. ;

El-Hajj, H. ;

Kohn, E. .

[6] pH sensors based on hydrogenated diamond surfaces -: art. no. 073504 [J].

Garrido, JA ;

Härdl, A ;

Kuch, S ;

Stutzmann, M ;

Williams, OA ;

Jackmann, RB .

APPLIED PHYSICS LETTERS, 2005, 86 (07) :1-3

[7] Molecular and biomolecular monolayers on diamond as an interface to biology [J].

Hamers, RJ ;

Butler, JE ;

Lasseter, T ;

Nichols, BM ;

Russell, JN ;

Tse, KY ;

Yang, WS .

[8] Effect of iodide ions on the hydrogen-terminated and partially oxygen-terminated diamond surface [J].

Kanazawa, H ;

Song, KS ;

Sakai, T ;

Nakamura, Y ;

Umezawa, H ;

Tachiki, M ;

Kawarada, H .

[9] Effect of electronic structures on electrochemical behaviors of surface-terminated boron-doped diamond film electrodes [J].

Liu, F. B. ;

Wang, J. D. ;

Liu, B. ;

Li, X. M. ;

Chen, D. R. .

[10] An ISFET-based penicillin sensor with high sensitivity, low detection limit and long lifetime [J].

Poghossian, A ;

Schöning, MJ ;

Schroth, P ;

Simonis, A ;

Lüth, H .

SENSORS AND ACTUATORS B-CHEMICAL, 2001, 76 (1-3) :519-526

← 1 2 3 →