Cloning and characterization of hGMEB1, a novel glucocorticoid modulatory element binding protein

被引：22

作者：

Thériault, JR ^{[1
]}

Charette, SJ ^{[1
]}

Lambert, H ^{[1
]}

Landry, J ^{[1
]}

机构：

[1] Univ Laval, Ctr Rech Cancerol, Hotel Dieu Quebec, Quebec City, PQ G1R 2J6, Canada

来源：

FEBS LETTERS | 1999年 / 452卷 / 03期

基金：

英国医学研究理事会;

关键词：

transcription factor; HSP27; glucocorticoid modulatory element binding protein;

D O I：

10.1016/S0014-5793(99)00634-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 21-bp element called glucocorticoid modulatory element (GME) modulates the glucocorticoid receptor-mediated responses via the binding of an as yet poorly characterized transacting complex of proteins containing the 88-kDa GMEB1 and the 67-kDa GMEB2, Using heat shock protein 27 (HSP27) as bait in the yeast two-hybrid assay, we cloned a 1.83-kb cDNA encoding a novel 573-amino acid protein called human GMEB1 (hGMEB1). hGMEB1 possesses a KDWK domain, contains sequences almost identical (36/38) to three tryptic peptides of rat GMEB1 and shares 38% identity with rat GMEB2, hGMEB1 is ubiquitously expressed as a 85-kDa protein in all cell lines and tissues examined, In vitro translated hGMEB1 bound specifically to GME oligonucleotides yielding a complex of similar size to the complex obtained using rat liver nuclear extracts. Both complexes were supershifted with an antibody specific to hGMEB1. Co-immunoprecipitation experiments confirmed the in vivo interaction of HSP27 with hGMEB1. (C) 1999 Federation of European Biochemical Societies.

引用

页码：170 / 176

页数：7

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