Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum

被引：577

作者：

Park, YongKeun ^{[3
]}

Diez-Silva, Monica ^{[4
]}

Popescu, Gabriel ^{[3
]}

Lykotrafitis, George ^{[4
]}

Choi, Wonshik ^{[3
]}

Feld, Michael S. ^{[3
]}

Suresh, Subra ^{[1
,2
]}

机构：

[1] MIT, Sch Engn, Cambridge, MA 02139 USA

[2] MIT, Harvard MIT Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[3] MIT, George R Harrison Spect Lab, Cambridge, MA 02139 USA

[4] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 37期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

erythrocyte; febrile temperature; malaria; mechanical properties; optical techniques;

D O I：

10.1073/pnas.0806100105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in A falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.

引用

页码：13730 / 13735

页数：6

共 30 条

[1]

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