Tomographic phase microscopy

被引：819

作者：

Choi, Wonshik

Fang-Yen, Christopher

Badizadegan, Kamran

Oh, Seungeun

Lue, Niyom

Dasari, Ramachandra R.

Feld, Michael S.

机构：

[1] MIT, GR Harrison Spectroscopy Lab, Cambridge, MA 02139 USA

[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Pathol, Boston, MA 02114 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 09期

关键词：

D O I：

10.1038/NMETH1078

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.

引用

收藏

页码：717 / 719

页数：3

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