Bacteria-based biosensor for determination of hydrogen peroxide

被引：4

作者：

Tai, GY ^{[1
]}

Wen, ML ^{[1
]}

Wang, CY ^{[1
]}

机构：

[1] YUNNAN UNIV,DEPT CHEM,KUNMING 650091,PEOPLES R CHINA

来源：

MICROCHEMICAL JOURNAL | 1996年 / 53卷 / 02期

关键词：

D O I：

10.1006/mchj.1996.0021

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A bacterial biosensor that immobilizes living bacteria of strain Bacillus subtilis AS 1.398 are coupled to a Clark-type oxygen electrode to determine hydrogen peroxide. The H2O2 is catalyzed by catalase from the bacteria to produce H2O and O-2. This sensor is advantageous in terms of linearity (10 to 2000 mu M H2O2), lifetime (65 days), and selectivity (no significant interference from some amino acids, metal ions, polyphenols, glucose, and ascorbic acid), especially because bacteria can be used as 2 catalase enzyme membrane that can reproduce itself. The correlation coefficient is 0.999. (C) 1996 Academic Press, Inc.

引用

收藏

页码：152 / 157

页数：6

相关论文

共 29 条

[1] LUMINOL CHEMILUMINESCENCE REACTION CATALYZED BY A MICROBIAL PEROXIDASE [J].

AKIMOTO, K ;

SHINMEN, Y ;

SUMIDA, M ;

ASAMI, S ;

AMACHI, T ;

YOSHIZUMI, H ;

SAEKI, Y ;

SHIMIZU, S ;

YAMADA, H .

ANALYTICAL BIOCHEMISTRY, 1990, 189 (02) :182-185

[2] KOHLRABI-BASED AMPEROMETRIC BIOSENSOR FOR HYDROGEN-PEROXIDE MEASUREMENT [J].

CHEN, L ;

LIN, MS ;

HARA, M ;

RECHNITZ, GA .

ANALYTICAL LETTERS, 1991, 24 (01) :1-14

[3] SPECTROFLUORIMETRIC DETERMINATION OF HYDROGEN-PEROXIDE BASED ON THE CATALYTIC EFFECT OF PEROXIDASE-LIKE MANGANESE TETRAKIS(SULPHOPHENYL) PORPHYRIN ON THE OXIDATION OF HOMOVANILLIC-ACID [J].

CI, YX ;

WANG, F .

ANALYTICA CHIMICA ACTA, 1990, 233 (02) :299-302

[4]

CI YX, 1990, ANAL CHIM ACTA, V237, P497

[5]

CI YX, 1989, GAODENG XUEXIAO HUAX, V10, P229

[6] SPECTROPHOTOMETRIC DETERMINATION OF HYDROGEN-PEROXIDE WITH LEUCO PATENT BLUE VIOLET [J].

CLAPP, PA ;

EVANS, DF .

ANALYTICA CHIMICA ACTA, 1991, 243 (02) :217-220

[7] CHEMICALLY IMMOBILIZED ENZYME ELECTRODES FOR HYDROGEN-PEROXIDE DETERMINATION [J].

COSGROVE, M ;

MOODY, GJ ;

THOMAS, JDR .

ANALYST, 1988, 113 (12) :1811-1815

[8] IMMOBILIZATION OF CATALASE ON MEMBRANES OF POLY(ETHYLENE)-G-CO-ACRYLIC ACID AND POLY(TETRAFLUOROETHYLENE)-G-CO-ACRYLIC ACID AND THEIR APPLICATION IN HYDROGEN-PEROXIDE ELECTROCHEMICAL SENSORS [J].

DASILVA, MA ;

GIL, MH ;

PIEDADE, AP ;

REDINHA, JS ;

BRETT, AMO ;

COSTA, JMC .

JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 1991, 29 (02) :269-274

[9]

DOMINGUEZ P, 1989, Analytical Proceedings, V26, P387

[10] FLUOROMETRIC FLOW-INJECTION DETERMINATION OF AQUEOUS PEROXIDES AT NANOMOLAR LEVEL USING MEMBRANE REACTORS [J].

HWANG, H ;

DASGUPTA, PK .

ANALYTICAL CHEMISTRY, 1986, 58 (07) :1521-1524