Degradation of PrPSc by keratinolytic protease from Nocardiopsis sp TOA-1

被引：31

作者：

Mitsuiki, Shinji

Hui, Zhao

Matsumoto, Daishi

Sakai, Masashi

Moriyama, Yasushi

Furukawa, Kensuke

Kanouchi, Hiroaki

Oka, Tatsuzo ^{[1
]}

机构：

[1] Kagoshima Univ, Dept Vet Physiol, Kagoshima 8900065, Japan

[2] Kyushu Sangyo Univ, Dept Appl Chem & Biochem, Fukuoka 8138503, Japan

[3] TOTO Ltd, Fundamental Res Ctr, Chigasaki, Kanagawa 2538577, Japan

[4] Kyushu Univ, Div Bioresource & Bioenvironm Sci, Fukuoka 8128581, Japan

[5] Kawasaki Med Sch, Dept Biochem, Kurashiki, Okayama 7010192, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 05期

关键词：

prion; alkaline protease; keratinase; Nocardiopsis sp;

D O I：

10.1271/bbb.70.1246

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A keratinolytic alkaline proteae (NAPase) from Nocardiopsis sp. TOA-1 degraded a scrapie prion without any chemical or physical treatment. Optimal temperature and pH were 60 degrees C and above pH 10.0. The scrapie prion was completely degraded within 3 min under optimal conditions.

引用

页码：1246 / 1248

页数：3

共 16 条

[1]

DIMCHEFF DE, 1997, TRENDS CELL BIOL, V7, P56

[2] COMPARATIVE-ANALYSIS OF SCRAPIE AGENT INACTIVATION METHODS [J].