A 3D finite element model of ventral furrow invagination in the Drosophila melanogaster embryo

被引:63
作者
Conte, Vito [1 ]
Munoz, Jose J. [2 ]
Miodownik, Mark [1 ]
机构
[1] Kings Coll London, Div Engn, Mat Res Grp, London WC2R 2LS, England
[2] Univ Polit Catalonia, Dept Appl Math III, Barcelona, Spain
基金
英国生物技术与生命科学研究理事会;
关键词
Invagination; Drosophila; Finite elasticity; Finite elements; Gastrulation; Active forces;
D O I
10.1016/j.jmbbm.2007.10.002
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The paper describes a mechanical model of epithelial tissue development in Drosophila embryos to investigate a buckling phenomenon called invagination. The finite element method is used to model this ventral furrow formation in 3D by decomposing the total deformation into two parts: an imposed active deformation, and an elastic passive deformation superimposed onto the latter. The model imposes as boundary conditions (i) a constant yolk volume and (ii) a sliding contact condition of the cells against the vitelline membrane, which is interpolated as a B-Spline surface. The active deformation simulates the effects of apical constriction and apico-basal elongation of cells. This set of local cellular mechanisms leads to global shape changes of the embryo which are associated with known gene expressions. Using the model we have tested different plausible hypotheses postulated to account for the mechanical behaviour of epithelial tissues. In particular, we conclude that only certain combinations of local cell shape change can successfully reproduce the invagination process. We have quantitatively compared the model with a 2D model and shown that it exhibits a more robust invagination phenomenon. The 3D model has also revealed that invagination causes a yolk flow from the central region to the anterior and posterior ends of the embryo, causing an accordion-like global compression and expansion wave to move through the embryo. Such a phenomenon cannot be described by 2D models. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:188 / 198
页数:11
相关论文
共 30 条
[1]  
[Anonymous], 2010, DYNAMIC ARCHITECTURE
[2]  
Bonet J., 1997, Nonlinear Continuum Mechanics For Finite Element Analysis
[3]   EMBRYONIC TISSUE MORPHOGENESIS MODELED BY FEM [J].
BRODLAND, GW ;
CLAUSI, DA .
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 1994, 116 (02) :146-155
[4]   Is mechano-sensitive expression of twist involved In mesoderm formation? [J].
Brouzés, E ;
Supatto, W ;
Farge, E .
BIOLOGY OF THE CELL, 2004, 96 (07) :471-477
[5]  
CLAUSI DA, 1993, DEVELOPMENT, V118, P1013
[6]   A PUTATIVE CELL SIGNAL ENCODED BY THE FOLDED GASTRULATION GENE COORDINATES CELL-SHAPE CHANGES DURING DROSOPHILA GASTRULATION [J].
COSTA, M ;
WILSON, ET ;
WIESCHAUS, E .
CELL, 1994, 76 (06) :1075-1089
[7]  
DAVIDSON LA, 1995, DEVELOPMENT, V121, P2005
[8]   Measurements of mechanical properties of the blastula wall reveal which hypothesized mechanisms of primary invagination are physically plausible in the sea urchin Strongylocentrotus purpuratus [J].
Davidson, LA ;
Oster, GF ;
Keller, RE ;
Koehl, MAR .
DEVELOPMENTAL BIOLOGY, 1999, 209 (02) :221-238
[9]   folded gastrulation, cell shape change and the control of myosin localization [J].
Dawes-Hoang, RE ;
Parmar, KM ;
Christiansen, AE ;
Phelps, CB ;
Brand, AH ;
Wieschaus, EF .
DEVELOPMENT, 2005, 132 (18) :4165-4178
[10]   Mechanical induction of twist in the Drosophila foregut/stomodeal primordium [J].
Farge, E .
CURRENT BIOLOGY, 2003, 13 (16) :1365-1377