Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses

被引：55

作者：

Chan, Jasper Fuk-Woo ^{[1
,2
,3
,4
]}

Choi, Garnet Kwan-Yue ^{[2
]}

Tsang, Alan Ka-Lun ^{[2
]}

Tee, Kah-Meng ^{[2
]}

Lam, Ho-Yin ^{[5
]}

Yip, Cyril Chik-Yan ^{[2
]}

To, Kelvin Kai-Wang ^{[1
,2
,3
,4
]}

Cheng, Vincent Chi-Chung ^{[2
]}

Yeung, Man-Lung ^{[2
]}

Lau, Susanna Kar-Pui ^{[1
,2
,3
,4
]}

Woo, Patrick Chiu-Yat ^{[1
,2
,3
,4
]}

Chan, Kwok-Hung ^{[2
]}

Tang, Bone Siu-Fai ^{[5
]}

Yuen, Kwok-Yung ^{[1
,2
,3
,4
]}

机构：

[1] Univ Hong Kong, State Key Lab Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China

[2] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China

[3] Univ Hong Kong, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China

[4] Univ Hong Kong, Carol Yu Ctr Infect, Hong Kong, Hong Kong, Peoples R China

[5] Hong Kong Sanat & Hosp, Dept Pathol, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 08期

关键词：

RESPIRATORY SYNDROME CORONAVIRUS; CELL-LINE; HCOV-EMC; VIRUS; DISEASE; TESTS; LNA; AMPLIFICATION; PERFORMANCE; VALIDATION;

D O I：

10.1128/JCM.01224-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.

引用

页码：2722 / 2726

页数：5

共 40 条

[11] Cross-reactive antibodies in convalescent SARS patients' sera against the emerging novel human coronavirus EMC (2012) by both immunofluorescent and neutralizing antibody tests
Chan, Kwok-Hung
Chan, Jasper Fuk-Woo
Tse, Herman
Chen, Honglin
Lau, Candy Choi-Yi
Cai, Jian-Piao
Tsang, Alan Ka-Lun
Xiao, Xincai
To, Kelvin Kai-Wang
Lau, Susanna Kar-Pui
Woo, Patrick Chiu-Yat
Zheng, Bo-Jiang
Wang, Ming
Yuen, Kwok-Yung
[J]. JOURNAL OF INFECTION, 2013, 67 (02) : 130 - 140
[12] Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43
Che, XY
Qiu, LW
Liao, ZY
Wang, YD
Wen, K
Pan, YX
Hao, W
Mei, YB
Cheng, VCC
Yuen, KY
[J]. JOURNAL OF INFECTIOUS DISEASES, 2005, 191 (12) : 2033 - 2037
[13] Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection
Cheng, Vincent C. C.
Lau, Susanna K. P.
Woo, Patrick C. Y.
Yuen, Kwok Yung
[J]. CLINICAL MICROBIOLOGY REVIEWS, 2007, 20 (04) : 660 - +
[14] Corman VM, 2012, EUROSURVEILLANCE, V17, P2
[15] Performance and clinical validation of the RealStar® MERS-CoV Kit for detection of Middle East respiratory syndrome coronavirus RNA
Corman, Victor Max
Oelschlaeger, Stephan
Wendtner, Clemens-Martin
Drexler, Jan Felix
Hess, Markus
Drosten, Christian
[J]. JOURNAL OF CLINICAL VIROLOGY, 2014, 60 (02) : 168 - 171
[16] Corman VM, 2012, EURO SURVEILL, V17
[17] MERS coronavirus: Data gaps for laboratory preparedness
de Sousa, Rita
Reusken, Chantal
Koopmans, Marion
[J]. JOURNAL OF CLINICAL VIROLOGY, 2014, 59 (01) : 4 - 11
[18] Herold J, 1993, Arch Virol Suppl, V7, P63
[19] Reverse transcriptase PCR diagnostic assay for the coronavirus associated with severe acute respiratory syndrome
Hui, RKH
Zeng, FY
Chan, CMN
Yuen, KY
Peiris, JSM
Leung, FCC
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) : 1994 - 1999
[20] Successful use of saliva without DNA extraction for detection of macrolide-resistant Mycoplasma pneumoniae DNA in children using LNA probe-based real-time PCR
Komatsu, Haruki
Tsunoda, Tomoyuki
Inui, Ayano
Sogo, Tsuyoshi
Fujisawa, Tomoo
Imura, Motoki
Tateno, Akihiko
[J]. JOURNAL OF INFECTION AND CHEMOTHERAPY, 2013, 19 (06) : 1087 - 1092

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