Multiplexed measurement of serum antibodies using an array biosensor

被引：38

作者：

Moreno-Bondi, MC

Taitt, CR

Shriver-Lake, LC

Ligler, FS ^{[1
]}

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

[2] Univ Complutense, Fac Quim, Dept Quim Analit, E-28040 Madrid, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2006年 / 21卷 / 10期

关键词：

vaccination; antibody production; biosensor; array; immunization; disease exposure; seroconversion;

D O I：

10.1016/j.bios.2005.12.018

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The array biosensor provides the capability for simultaneously measuring titers of antibody against multiple antigens. Human antibodies against four different targets, tetanus toxin, diphtheria toxin, staphylococcal enterotoxin B (SEB) and hepatitis 13, were measured simultaneously in sera from eight different donors in a single assay and titers were determined. The assays could measure amounts of bound antibody as low as approximately 100 fg. Each individual serum exhibited a different pattern of reactivity against the four target antigens. Applications of this biosensor capability include monitoring for exposure to pathogens and for efficacy of vaccination. (c) 2006 Elsevier B.V. All rights reserved.

引用

收藏

页码：1880 / 1886

页数：7

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