Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy

被引：65

作者：

Li, LW

Wetzel, S

Plückthun, A

Fernandez, JM ^{[1
]}

机构：

[1] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA

[2] Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland

来源：

BIOPHYSICAL JOURNAL | 2006年 / 90卷 / 04期

关键词：

D O I：

10.1529/biophysj.105.078436

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Using single-molecule atomic force microscopy, we find that a protein consisting of six identical ankyrin repeat units flanked by N- and C- terminal modules (N6C) unfolds in a stepwise, unit-by-unit fashion under a mechanical force. Stretching a N6C molecule results in a sawtooth pattern fingerprint, with as many as six peaks separated by similar to 10 nm and an average unfolding force of 50 +/- 20 pN. Our results demonstrate that a stretching force can unfold multiple repeat units individually in a single protein molecule, despite extensive hydrophobic interactions between adjacent units.

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页码：L30 / L32

页数：3

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