SIMULTANEOUS DETERMINATION OF CREATININE AND BIOLOGICAL INDICATORS OF EXPOSURE TO TOLUENE, STYRENE AND XYLENES IN URINE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

被引：7

作者：

Antunes, Marina Venzon ^{[1
]}

Marques Patuzzi, Ana Luiza ^{[1
]}

Linden, Rafael ^{[1
]}

机构：

[1] Ctr Univ Feevale, Inst Ciencias Saude, Curso Ciencias Farmaceut, BR-93352000 Novo Hamburgo, RS, Brazil

来源：

QUIMICA NOVA | 2008年 / 31卷 / 07期

关键词：

HPLC-DAD; organic solvent exposure; biological monitoring;

D O I：

10.1590/S0100-40422008000700045

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple liquid chromatographic method for the simultaneous determination of creatinine, hippuric acid, mandelic acid, phenylglyoxylic acid and o, m and p-methylhippuric acids was developed and validated. Sample preparation was only dilution with water (1: 10), followed by centrifugation. Analysis was performed in a reversed phase column (Lichrospher RP 8ec), 250 x 4.0 mm, with isocratic elution with phosphate buffer pH 2.3 and acetonitrile (90: 10, v/v). The method presents adequate linearity, precision and accuracy and allows the simultaneous determination of the biomarkers of exposure to toluene, xylene and styrene together with creatinine, reducing cost and laboratory time.

引用

收藏

页码：1865 / 1868

页数：4

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