Synthesis of polyallylamine derivatives and their use as gene transfer vectors in vitro

被引:85
作者
Boussif, O
Delair, T
Brua, C
Veron, L
Pavirani, A
Kolbe, HVJ
机构
[1] Transgene SA, F-67082 Strasbourg, France
[2] Ecole Normale Super Lyon, Unite Mixte CNRS BioMerieux, F-69364 Lyon 07, France
关键词
D O I
10.1021/bc9900439
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cationic polymers possessing primary amine groups are inefficient in transferring nucleic acids into eukaryotic cells. With appropriate chemical modification, namely glycolylation of the amine groups of polylysine and polyallylamine, the actual number of free amino groups was decreased, hydrophilic residues were introduced, and the cytotoxicity of both polymers decreased significantly. Furthermore, in the case of polyallylamine, its ability to mediate gene transfer into cells increased by several orders of magnitude. Transfection efficiency was found to be dependent on the substitution level of amino groups and reached highest levels in the presence of lysosomotropic and/or fusogenic agents. At optimal conditions, glycolylated PAM was shown to be as efficient as the linear polyethylenimine of 22 kDa.
引用
收藏
页码:877 / 883
页数:7
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