Interactions of antitumor metallodrugs with serum proteins: Advances in characterization using modern analytical methodology

Serum proteins play a critical role in the transport, delivery, and mechanism of action of anticancer metal-containing drugs. Efficient and conventional analytical methods capable of providing a better understanding of such protein functions have been a long sought after goal for antitumor metallodrug developers. This review highlights the various aspects of recent protein - metallodrug binding studioes and critically examines the potential and utilization of conventional and advanced analytical techniques in the growing-in-importance area. The most frequently used techniques are compared in terms of merits and limitations in an ordering that reflects not only the respective literature covering but rather their relative importance for further progress in the field.