Evaluation of the molecular interactions of fluorinated amino acids with native polypepticles

被引：52

作者：

Jäckel, C ^{[1
]}

Seufert, W ^{[1
]}

Thust, S ^{[1
]}

Koksch, B ^{[1
]}

机构：

[1] Univ Leipzig, Dept Organ Chem, D-04103 Leipzig, Germany

来源：

CHEMBIOCHEM | 2004年 / 5卷 / 05期

关键词：

amino acids; coiled-coil peptides; fluorine; helical structures; protein modifications; self replication;

D O I：

10.1002/cbic.200300840

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One fluorine can make a difference. Investigation of the properties of fluorinated amino acids in a native-polypeptide environment increases our ability to use this class of non-natural amino acids for protein engineering. Two fluorinated amino acids (see figure) were incorporated into different positions of a heptad repeat motif, and their influence on coiled-coil peptide formation was studied. The screens introduced here are sufficiently sensitive to even detect the difference of one single fluorine atom in the side chain of an amino acid.

引用

页码：717 / 720

页数：4

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