Cloning and characterization of new bioluminescent proteins

被引：18

作者：

Szent-Gyorgyi, C ^{[1
]}

Ballon, BT ^{[1
]}

Dagnal, E ^{[1
]}

Bryan, B ^{[1
]}

机构：

[1] Prolume Ltd, Pittsburgh, PA 15238 USA

来源：

BIOMEDICAL IMAGING: REPORTERS, DYES, AND INSTRUMENTATION, PROCEEDINGS OF | 1999年 / 3600卷

关键词：

luciferase; green fluorescent protein (GFP); coelenterazine; evolution; energy transfer;

D O I：

10.1117/12.351015

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Over the past two years Prolume has undertaken a comprehensive program to clone luciferases and associated "green fluorescent proteins" (GFPs) from marine animals that use coelenterazine as the luciferin. To date we have cloned several bioluminescent proteins, including two novel copepod luciferases and two anthozoan GFPs. These four proteins have sequences that differ greatly from previously cloned analogous proteins; the sequence diversity apparently is due to independent evolutionary origins (copepod luciferases) and unusual evolutionary constraints (anthozoan GFPs). Thus coelenterazine-based bioluminescent systems may also manifest a variety of useful properties. We discuss from this taxonomic perspective the initial biochemical and spectral characterization of out cloned proteins. Emphasis is placed on the anthozoan luciferase-GFP systems, whose efficient resonance energy transfer has elicited much current interest.

引用

页码：4 / 11

页数：8

共 16 条

[11] MOLECULAR EVOLUTION OF THE CA2+-BINDING PHOTOPROTEINS OF THE HYDROZOA [J].