Enzymatic hydrolysis of the conjugate of vitamin D and related compounds

The monoglucuronides of vitamin D, 25-hydroxtvitamin D and the corresponding pro-forms were subjected to enzymatic hydrolysis using beta-glucuronidase, and substrate specificities were found in the examined enzymes originating from different sources, which were determined using reversed-phase high-performance liquid chromatography with UV detection. The enzymatic hydrolysis of the corresponding monosulfates was also examined using the same system. (C) 1997 Elsevier Science B.V.