Identification of furfural as a key toxin in lignocellulosic hydrolysates and evolution of a tolerant yeast strain

被引:215
作者
Heer, Dominik [1 ]
Sauer, Uwe [1 ]
机构
[1] ETH, Inst Mol Syst Biol, CH-8093 Zurich, Switzerland
来源
MICROBIAL BIOTECHNOLOGY | 2008年 / 1卷 / 06期
关键词
D O I
10.1111/j.1751-7915.2008.00050.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The production of fuel ethanol from low-cost lignocellulosic biomass currently suffers from several limitations. One of them is the presence of inhibitors in lignocellulosic hydrolysates that are released during pre-treatment. These compounds inhibit growth and hamper the production of ethanol, thereby affecting process economics. To delineate the effects of such complex mixtures, we conducted a chemical analysis of four different real-world lignocellulosic hydrolysates and determined their toxicological effect on yeast. By correlating the potential inhibitor abundance to the growth-inhibiting properties of the corresponding hydrolysates, we identified furfural as an important contributor to hydrolysate toxicity for yeast. Subsequently, we conducted a targeted evolution experiment to improve growth behaviour of the half industrial Saccharomyces cerevisiae strain TMB3400 in the hydrolysates. After about 300 generations, representative clones from these evolved populations exhibited significantly reduced lag phases in medium containing the single inhibitor furfural, but also in hydrolysate-supplemented medium. Furthermore, these strains were able to grow at concentrations of hydrolysates that effectively killed the parental strain and exhibited significantly improved bioconversion characteristics under industrially relevant conditions. The improved resistance of our evolved strains was based on their capacity to remain viable in a toxic environment during the prolonged, furfural induced lag phase.
引用
收藏
页码:497 / 506
页数:10
相关论文
共 47 条
[41]   ANOTHER EXPLANATION FOR THE TOXICITY OF FERMENTATION ACIDS AT LOW PH - ANION ACCUMULATION VERSUS UNCOUPLING [J].
RUSSELL, JB .
JOURNAL OF APPLIED BACTERIOLOGY, 1992, 73 (05) :363-370
[42]   Fermentation performance of engineered and evolved xylose-fermenting Saccharomyces cerevisiae strains [J].
Sonderegger, M ;
Jeppsson, M ;
Larsson, C ;
Gorwa-Grauslund, MF ;
Boles, E ;
Olsson, L ;
Spencer-Martins, I ;
Hahn-Hägerdal, B ;
Sauer, U .
BIOTECHNOLOGY AND BIOENGINEERING, 2004, 87 (01) :90-98
[43]  
STEIN SE, 2005, 69 NIST
[44]   Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae:: current status [J].
van Maris, Antonius J. A. ;
Abbott, Derek A. ;
Bellissimi, Eleonora ;
van den Brink, Joost ;
Kuyper, Marko ;
Luttik, Marijke A. H. ;
Wisselink, H. Wouter ;
Scheffers, W. Alexander ;
van Dijken, Johannes P. ;
Pronk, Jack T. .
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2006, 90 (04) :391-418
[45]   THE VALUE OF ELECTROPHORETIC FINGERPRINTING AND KARYOTYPING IN WINE YEAST BREEDING PROGRAMS [J].
VANDERWESTHUIZEN, TJ ;
PRETORIUS, IS .
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 1992, 61 (04) :249-257
[46]   EFFECT OF BENZOIC-ACID ON METABOLIC FLUXES IN YEASTS - A CONTINUOUS-CULTURE STUDY ON THE REGULATION OF RESPIRATION AND ALCOHOLIC FERMENTATION [J].
VERDUYN, C ;
POSTMA, E ;
SCHEFFERS, WA ;
VANDIJKEN, JP .
YEAST, 1992, 8 (07) :501-517
[47]   Generation of the improved recombinant xylose-utilizing Saccharomyces cerevisiae TMB 3400 by random mutagenesis and physiological comparison with Pichia stipitis CBS 6054 [J].
Wahlbom, CF ;
van Zyl, WH ;
Jönsson, LJ ;
Hahn-Hägerdal, B ;
Otero, RRC .
FEMS YEAST RESEARCH, 2003, 3 (03) :319-326