Oxidative damage to mitochondrial DNA in Huntington's disease parietal cortex

被引：164

作者：

Polidori, MC

Mecocci, P

Browne, SE

Senin, U

Beal, MF

机构：

[1] Perugia Univ Hosp, Inst Gerontol & Geriatr, Perugia, Italy

[2] Massachusetts Gen Hosp, Neurol Serv, Neurochem Lab, Boston, MA 02114 USA

[3] Harvard Univ, Sch Med, Boston, MA USA

来源：

NEUROSCIENCE LETTERS | 1999年 / 272卷 / 01期

关键词：

Huntington's disease; mitochondria; DNA; oxidative stress; Free radicals; 8-Hydroxy-2-deoxyguanosine;

D O I：

10.1016/S0304-3940(99)00578-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Oxidative damage to DNA may play a role in both normal aging and in neurodegenerative diseases. Using a sensitive high-performance liquid chromatography (HPLC) assay, we examined concentrations of 8-hydroxy-2-deoxyguanosine (OH(8)dG) in mitochondrial DNA (mtDNA) isolated from frontal and parietal cerebral cortex and from cerebellum in 22 Huntington's disease (HD) patients and 15 age-matched normal controls. A significant increase in OH(8)dG in mtDNA of parietal cortex was found in HD patients as compared with controls, while there were no significant changes in frontal cortex or cerebellum. The present findings are consistent with regionally specific oxidative damage in HD, which may be a further evidence of a metabolic defect. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：53 / 56

页数：4