Determination of dissociation constant and concentration of an anti-DNA antibody by using the light-addressable potentiometric sensor

被引：6

作者：

Dill, K

Fraser, C

Blomdahl, JA

Olson, JD

机构：

[1] Molecular Devices Corporation, Sunnyvale, CA 94089

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1996年 / 31卷 / 1-2期

关键词：

anti-DNA antibody; dissociation constant; biotinylated oligonucleotide; light-addressable potentiometric sensor;

D O I：

10.1016/0165-022X(95)00023-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The most sensitive assay possible is desired for quantitation of total DNA and DNA probe binding. To reduce background signal, which may reduce assay sensitivity, it is desirable to employ low antibody concentrations. This requires the use of a specific antibody with a high affinity for DNA.

引用

收藏

页码：17 / 21

页数：5

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