Increased expression of interleukin-16 in bronchial mucosa of subjects with atopic asthma

被引:120
作者
Laberge, S
Ernst, P
Ghaffar, O
Cruikshank, WW
Kornfeld, H
Center, DM
Hamid, Q
机构
[1] MCGILL UNIV,MEAKINS CHRISTIE LABS,MONTREAL,PQ,CANADA
[2] MCGILL UNIV,MONTREAL GEN HOSP,MONTREAL,PQ H3G 1A4,CANADA
[3] BOSTON UNIV,SCH MED,CTR PULM,BOSTON,MA 02118
关键词
D O I
10.1165/ajrcmb.17.2.2750
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Asthma is characterized by the presence of activated CD4(+) cells in the airways. We hypothesized that the newly characterized cytokine interleukin (IL)-16 is involved in the pathogenesis of asthma through its ability to selectively induce CD4(+) cell recruitment within the inflamed bronchial wall. We investigated the expression of IL-16 in bronchial biopsies obtained from subjects with mild asthma (n = 10), atopic nonasthmatic individuals (n = 6), and normal control subjects (n = 10). Cryostat sections from 4% paraformaldehyde-fixed fiberoptic bronchial biopsies were immunostained using a specific antibody that recognizes human IL-16. IL-16 mRNA expression was determined by in situ hybridization. IL-16 immunoreactivity and mRNA were demonstrated mainly in bronchial epithelial cells in all subjects. IL-16 immunoreactivity and IL-16 mRNA expression within the epithelium were significantly higher in bronchial biopsies obtained from asthmatic subjects as compared to both atopic nonasthmatic and normal controls (P < 0.001). The numbers of subepithelial IL-16 immunoreactive cells and lL-16 mRNA-positive cells were also ter in the bronchial biopsies obtained from asthmatic subjects as compared to both atopic nonasthmatic and normal controls (P < 0.001). Epithelial expression of IL-16 immunoreactivity and mRNA correlated with the CD4(+) cell infiltration (r(2) = 0.70, P < 0.001). There were significant associations between epithelial and subepithelial IL-16 immunoreactivity and airway responsiveness to methacholine. This study demonstates that IL-16 is expressed in airway tissues, particularly in the epithelial cells: and that upregulation of its expression is a feature of allergic asthma. These results suggest an in vivo role for IL-16 in the pathogenesis of asthma, possibly through the recruitment of CD4(+) cells, and support the increasing evidence for the participation of epithelial cells in regulating inflammatory responses.
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收藏
页码:193 / 202
页数:10
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