Differential expression of opioid receptor genes in human lymphoid cell lines and peripheral blood lymphocytes

被引:71
作者
Wick, MJ
Minnerath, SR
Roy, S
Ramakrishnan, S
Loh, HH
机构
[1] UNIV MINNESOTA,SCH MED,DEPT PHARMACOL,MINNEAPOLIS,MN 55455
[2] UNIV MINNESOTA,SCH MED,DEPT SURG,MINNEAPOLIS,MN 55455
关键词
delta opioid receptor; kappa opioid receptor; human MOLT-4 T-leukemic cell line; human CEM-3 T-leukemic cell line; human peripheral blood lymphocytes; northern analysis; reverse transcription polymerase chain reaction;
D O I
10.1016/0165-5728(95)00144-1
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The existence of receptors for opioid compounds on cells of the immune system has long been hypothesized, but has been very difficult to demonstrate unequivocally. We have used reverse-transcription polymerase chain reaction to obtain cDNA clones from the human MOLT-4 and CEM-3 T-leukemic cell lines Which are nearly identical to portions of the delta and kappa opioid receptor cDNAs recently isolated from human brain and placenta, respectively. Northern analyses with riboprobes derived from the delta and kappa opioid receptor clones indicate these sequences are expressed at low levels in human peripheral blood lymphocytes and in several human lymphoid cell lines. Sequences corresponding to the mu opioid receptor cDNA were not detected in this study. These results suggest that delta and kappa opioid receptors may be responsible for mediating some direct effects of opioids in immune cells.
引用
收藏
页码:29 / 36
页数:8
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